Highly purified native human Retinol Binding Protein.
Product Specification
| Alternative Name: | Retinol binding protein |
| |
| MW: | ~21kDa |
| |
| Source: | Purified from urine of patients with chronic renal tubular proteinuria. |
| |
| UniProt ID: | P04916 (RBP4) |
| |
| Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
| |
| Purity: | ≥95% (SDS-PAGE) |
| |
| Purity Detail: | Purified by multi-step chromatography. |
| |
| Applications: | WB
|
| |
| Application Notes: | Western blot control. |
| |
| Shipping: | Shipped on Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| |
| Scientific Background: | Retinol is the alcohol form of vitamin A. Derivatives of retinol replacing the alcohol functional group form a class of molecules knows as retinoids which play critical roles in vision, embryonic gene expression, and bone and skin health.
Retinol binding protein binds retinol and regulates its transport and metabolism. RBP4 is the sole molecule used for transport of retinol in blood. In serum it is normally complexed to transerytrhin, which facilitates uptake by the glomeruli membranes, but a fraction remains unbound and is absorbed by renal proximal tubules. Injury to the proximal tubules is associated with renal tubulointerstitial nephritis caused by environmental allergens or infection, and can be a secondary factor of glomerulopathies. Injury can result in failure to absorb RBP, which is then lost to the urine as a variety of processed subspecies. As such, elevated levels of RBP in the urine can be used as an indicator or proximal tubular injury, and a broader indicator of kidney disease. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Western blot analysis of RBP. Lane 1: MW Marker, Lane 2: 100 ng, Lane 3: 200 ng, Lane 4: 500 ng. Probed with RBP polyclonal antibody.
SDS-PAGE analysis of RBP. Lane 1: MW Marker, Lane 2: 1 µg, Lane 3: 2 µg, Lane 4: 5 µg.
Please mouse over
General Literature References
Analysis of molecular forms of urine Retinol-Binding Protein in Fanconi Syndrome and design of an accurate immunoassay.: K.A. Burling, et al.; Clin Chim Acta.
413, 483 (2012),
Abstract;
Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by a NADPH Oxidase- and Nuclear Factor Kappa B-dependent and Retinol-Independent Mechanism.: K.M. Farjo, et al.; Mol Cell Biol.
32, 5103 (2012),
Abstract;
Assessment of urinary retinol-binding protein as an index of proximal tubular injury.: A. M. Bernard, et al.; Clin Chem
33, 775 (1987),
Abstract;
Related Products
