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Cpn10 (human), (recombinant)

ADI-SPP-110-D 50 µg 270.00 USD
ADI-SPP-110-F 200 µg 650.00 USD
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Product Details

Alternative Name:HSP10, HSPE1, GROES, Heat shock protein 10, Chaperonin 10
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Source:Produced in E. coli.
UniProt ID:P61604
Formulation:Liquid. In 20mM TRIS, pH 8.0, containing 150mM sodium chloride.
Purity:≥90% (SDS-PAGE; Western blot)
Purity Detail:Purified by multi-step chromatography.
Applications:ELISA, WB
Application Notes:Western blot control.
Shipping:Dry Ice
Long Term Storage:-80°C
Scientific Background:Chaperonin 10 (Cpn 10) is the ~10kD mammalian equivalent to GroES of E. coli. Chaperonins play fundamental roles in the folding, assembly, and translocation of other proteins. Chaperonin function in vivo requires two distinct family members, Cpn60 and Cpn10, both of which are also present in chloroplasts and mitochondria. The best studied Cpn60 and Cpn10, are the GroEL and GroES proteins ofE. coli, respectively. Cpn10 and Cpn60 both exhibit a sevenfold axis of symmetry and function as a team in the protein folding and assembly processes. Chaperonin 10 has been located in human platelets but more importantly it may also be present in human maternal serum. It has been reported that human Cpn10 is identical to early pregnancy factor (EPF), which is involved in control over cell growth and development. This identification suggests that Cpn10 may act like a hormone in stressful situations such as pregnancy.
Regulatory Status:RUO - Research Use Only
Cpn10 (human), (recombinant) Western blot
Western Blot analysis: Lane 1: MW marker, Lane 2: Cpn10 Recombinant Human Protein, Lane 3: HeLa lysate (Prod. No. ADI-LYC-HL100) probed with Cpn10 polyclonal antibody (Prod. No. ADI-SPA-110).
Cpn10 (human), (recombinant) SDS-PAGE
SDS-PAGE analysis: Lane 1: MWM, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg Cpn10 Recombinant Human Protein.
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Cpn10 (human), (recombinant) Western blot Cpn10 (human), (recombinant) SDS-PAGE

Product Literature References

Mitochondrial Chaperonin HSP60 Is the Apoptosis-Related Target for Myrtucommulone: K. Wiechmann, et al.; Cell Chem. Biol. 24, 614 (2017), Abstract;
Serum immunoglobulin g antibodies to chlamydial heat shock protein 60 but not to human and bacterial homologs are associated with coronary artery disease: G.I. Byrne, et al. ; Circulation 106, 1659 (2002), Application(s): EIA , Abstract;

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