Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

BAG-1M (human), (recombinant) (His-tag)

ADI-APR-400-0200 200 µg 530.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:BCL2-associated athanogene
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
MW:~46kDa (~51kDa observed with His-tag)
Source:Produced in E. coli. Human BAG-1M is fused at the N-terminus to a His-tag.
UniProt ID:Q99933
Formulation:Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 1mM DTT, and 1% glycerol.
Purity:≥80% (SDS-PAGE; Western Blot)
Purity Detail:Purified by multi-step chromatography.
Application Notes:Western blot control.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Scientific Background:BAG-1 is a Bcl-2-binding protein which lacks sequence homology with Bcl-2 and its related proteins, and functions to enhance the anti-apoptotic effects of Bcl- 2. BAG-1 is expressed as three major functionally distinct and differentially localized isoforms, which originate from a single mRNA. BAG-1S, 36 kDa, typically found in cytoplasm, generally the most abundant isoform in normal cells; BAG-1M, 46 kDa, found in both the nucleus and cytoplasm and under certain conditions, it can translocate from cytoplasm to nucleus; and BAG-1L, 50 kDa, nuclear localized, involved in regulating transcription. BAG-1 binds to the intracellular domain of the hepatocyte growth factor (HGF) and platelet-derived growth factor (PDGF) receptors, enhancing growth factor mediated protection from apoptosis. BAG-1 binds to the ATPase domain of Hsc70/Hsp70 to modulate its chaperone activity, in-part regulating Raf-1 activity and the assembly of glucocorticoid receptors.
Regulatory Status:RUO - Research Use Only
BAG-1M (human), (recombinant) (His-tag) ELISA
ELISA analysis of interaction between BAG-1M (human), (recombinant) (His-tag) (Prod. No. ADI-APR-400), BAG-1S (human), (recombinant) (His-tag) (GST-tag) (Prod. No. ADI-APR-401) and HSP70 (Prod. No. ADI-ESP-555). 2-Fold serial dilution of HSP70 from 38 μg/ml down to 0.58 ng/ml was incubated with plate pre-coated with 10μg of BAG-1M, BAG-1S or BSA. Bound HSP70 was probed with HSP70 mAb (Prod. No. ADI-SPA-810) followed by goat anti-mouse IgG, HRP-conjugated (Prod. No. ADI-SAB-100), and detected by TMB.
BAG-1M (human), (recombinant) (His-tag) Western blot
Western Blot analysis: Lane 1: MW marker, Lane 2: BAG-1M (human), (recombinant) (His-tag) (Prod. No. ADI-APR-400), Lane 3: BAG-1S (human), (recombinant) (His-tag) (GST-tag) (Prod. No. ADI-APR-401) probed with BAG-1, pAb (Prod. No. ADI-905-735).
BAG-1M (human), (recombinant) (His-tag) SDS-PAGE
SDS-PAGE analysis of BAG-1M (human), (recombinant) (His-tag) (Prod. No. ADI-APR-400): Lane 1: MW marker, Lane 2: 0.5μg, Lane 3: 1μg BAG-1M.
Please mouse over
BAG-1M (human), (recombinant) (His-tag) ELISA BAG-1M (human), (recombinant) (His-tag) Western blot BAG-1M (human), (recombinant) (His-tag) SDS-PAGE

Product Literature References

The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling: A. Mashukova, et al.; J. Cell Sci. 127, 3568 (2014), Abstract; Full Text

General Literature References

BAG-1: a multi-functional pro-survival molecule: P.A. Townsend, et al.; Int. J. Biochem. Cell. Biol. 37, 251 (2005), Abstract;
Differential effects of the hsp70-binding protein BAG-1 on glucocorticoid receptor folding by the hsp90-based chaperone machinery: K.C. Kanelakis, et al.; J. Biol .Chem. 274, 34134 (1999), Abstract;
BAG-1 modulates the chaperone activity of Hsp70/Hsc70: S. Takayama, et al.; EMBO J. 16, 4887 (1997), Abstract;
Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1: H.G. Wang, et al.; PNAS U.S.A. 93, 7063 (1996), Abstract;
HGF receptor associates with the anti-apoptotic protein BAG-1 and prevents cell death: A. Bardelli, et al.; EMBO J. 15, 6205 (1996), Abstract;
Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity: S. Takayama, et al.; Cell 80, 279 (1995), Abstract;

Related Products

Bag-1 polyclonal antibody 

WB | Print as PDF
ADI-905-735-100 100 µg 396.00 USD
Do you need bulk/larger quantities?

HSP70/HSP72 monoclonal antibody (C92F3A-5) 

Purified from ascites., ELISA, Flow Cytometry, ICC, IF, IHC, IP, WB, Electron microscopy | Print as PDF
ADI-SPA-810-D 50 µg 212.00 USD
ADI-SPA-810-F 200 µg 443.00 USD
ADI-SPA-810-J 1 mg 1,861.00 USD
Do you need bulk/larger quantities?

Goat anti-mouse IgG F(ab')2, polyclonal antibody (HRP conjugate) 

ELISA, IHC, WB | Print as PDF
ADI-SAB-100-J 1 ml 130.00 USD
Do you need bulk/larger quantities?

HSP70 (low endotoxin) (human), (recombinant) 

Low endotoxin HSP70 recombinant protein.
Produced in E. coli., ≥90% (SDS-PAGE; Western blot), ELISA, WB, Activity assay, in vitro Assay | Print as PDF
ADI-ESP-555-D 50 µg 381.00 USD
ADI-ESP-555-F 200 µg 951.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
BAG Recombinant protein
By catalog section:


Technical Service
Customer Service

Related Literature

Monitor programmed cell death from membrane to nucleus
Monitor programmed cell death from membrane to nucleus
Download as PDF

All new literature pieces

Recommend this page

Keep in touch

©2021 Enzo Life Sciences, Inc.,