Product Specification
Alternative Name: | MORT1, Fas (TNFRSF6)-associated via death domain |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to a portion of human FADD. |
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UniProt ID: | Q13158 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human, Rat Monkey
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Applications: | ICC, IHC (PS), WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~28kDa by Western blot. |
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Purity Detail: | Peptide affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Fas-associating death domain containing protein (FADD)/MORT1 serves as a signal transducer of Fas-induced apoptosis. Through its amino-terminal death effector domain (DED), FADD binds to cytosolic proteins such as TRADD, a cytosolic death domaincontaining protein that asssociates with the cytosolic portion of TNF receptor, or MACH/FLICE/caspase-8, a protease with sequence homology to ICE-CED-3 protease family, in order to transduce the death signal. |
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Regulatory Status: | RUO - Research Use Only |
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IHC Analysis: Human kidney tissue stained with FADD, pAb at 10µg/ml.
Western blot analysis: Lane 1: MW marker, Lane 2: HeLa.
IHC Analysis: Human placenta tissue stained with FADD, pAb at 10µg/ml.
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Product Literature References
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
15, 909 (2018),
Abstract;
Hepatocyte Fas-associating death domain protein/mediator of receptor-induced toxicity (FADD/MORT1) levels increase in response to pro-apoptotic stimuli: T.R. Billiar, et al. ; J. Biol. Chem.
277, 38855 (2002),
Application(s): ICC, WB using rat samples,
Abstract;
B cell receptor cross-linking triggers a caspase-8-dependent apoptotic pathway that is independent of the death effector domain of Fas-associated death domain protein: A. Vazquez, et al. ; J. Immunol.
167, 733 (2001),
Application(s): WB using human samples,
Abstract;
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