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FADD polyclonal antibody

ADI-AAP-210-D 50 µg 227.00 USD
ADI-AAP-210-F 200 µg 433.00 USD
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Product Details

Alternative Name:MORT1, Fas (TNFRSF6)-associated via death domain
Immunogen:Synthetic peptide corresponding to a portion of human FADD.
UniProt ID:Q13158
Source:Purified from rabbit serum.
Species reactivity:Human, Rat
Applications:ICC, IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~28kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Fas-associating death domain containing protein (FADD)/MORT1 serves as a signal transducer of Fas-induced apoptosis. Through its amino-terminal death effector domain (DED), FADD binds to cytosolic proteins such as TRADD, a cytosolic death domaincontaining protein that asssociates with the cytosolic portion of TNF receptor, or MACH/FLICE/caspase-8, a protease with sequence homology to ICE-CED-3 protease family, in order to transduce the death signal.
Regulatory Status:RUO - Research Use Only
FADD polyclonal antibody Immunohistochemistry
IHC Analysis: Human kidney tissue stained with FADD, pAb at 10µg/ml.
FADD polyclonal antibody Western blot
Western blot analysis: Lane 1: MW marker, Lane 2: HeLa.
FADD polyclonal antibody Immunohistochemistry
IHC Analysis: Human placenta tissue stained with FADD, pAb at 10µg/ml.
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FADD polyclonal antibody Immunohistochemistry FADD polyclonal antibody Western blot FADD polyclonal antibody Immunohistochemistry

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Hepatocyte Fas-associating death domain protein/mediator of receptor-induced toxicity (FADD/MORT1) levels increase in response to pro-apoptotic stimuli: T.R. Billiar, et al. ; J. Biol. Chem. 277, 38855 (2002), Application(s): ICC, WB using rat samples, Abstract;
B cell receptor cross-linking triggers a caspase-8-dependent apoptotic pathway that is independent of the death effector domain of Fas-associated death domain protein: A. Vazquez, et al. ; J. Immunol. 167, 733 (2001), Application(s): WB using human samples, Abstract;

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