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Immunoassay
& Assay Kits

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Browse our complete Assay Kits range

Flexible Formats

ELISA Kits

Enzo Life Sciences offers hundreds of immunoassay and enzyme activity assay kits in a variety of formats to service the biomarker and drug discovery assay markets. Our assay offering includes both competitive immunoassays for peptides and small molecules such as cyclic nucleotides and eicosanoids, and immunometric “sandwich” ELISA format assays for large protein analytes including cytokines, cell stress proteins, and signaling pathway regulators. As scientists and manufacturers of kits, we understand the critical nature of your research. Each of our assay kits undergoes rigorous testing to ensure high precision and accuracy while delivering the sensitivity and specificity to detect biologically relevant levels of analyte. Our industry-proven manufacturing capabilities guarantee you will obtain reproducible results, day-after-day and lot-after-lot.

Our decades of experience in the design and manufacture of active enzymes and their substrates supports development of an ever-expanding portfolio of biochemical assays. Our menu of scalable enzyme activity assays includes kits for epigenetic modulation (HDAC, HAT, Sirtuin), matrix metalloproteinases (MMPs) and caspase activity, as well as phosphospecific antibody-based kinase assays. These assays exemplify the synergy in our capabilities, assembling small peptide, active recombinant protein synthesis, fluorescent labeling and detection technologies, and antibody development into robust, reproducible assay kits.

Featured Literature

Product Flyers
Flexible Formats for Biomarker Detection, Cell Analysis, and Enzyme Activity
Flexible Formats for Biomarker Detection, Cell Analysis, and Enzyme Activity 24-Oct-12
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Immunoassays ELISA Kits

Detect Small And Large Analytes Accurately & Efficiently

  • Competitive & immunometric formats
  • Multi-plate packs available
  • Color coded reagents reduce user error
  • Amp’d™ Technology to improve ELISA sensitivity

Our catalog of nearly 300 ELISA kits includes sensitive, specific assays for relevant markers of cell viability, signaling pathways, steroid and peptide hormones, inflammation, and more. In addition to traditional antibody pair based immunometric assays, our scientists specialize in the particularly challenging production of high-specificity competitive ELISAs for detection of small molecules. Over 20 years of assay development experience and state-of-the-art manufacturing facilities ensure time-tested reproducibility.


Competetive ELISA Format for Small Molecules & Peptides

Immunometric ELISA Format for Large Molecules

 

CELLestial® Assays

Visualize Cellular Responses

  • Novel assays for autophagy, aggregation, and multi-drug resistance
  • Increased photo-stability and permeability
  • Multiplex compatible (GFP-Certified®) assays

Our CELLestial® portfolio of fluorescent probes and assay kits for cellular analysis provides a complete set of tools for monitoring cell viability, proliferation, death, oxidative stress and toxicology. Our dye-based assays have been optimized for the most demanding imaging applications including confocal microscopy, wide-field fluorescence microscopy, flow cytometry, and high content screening, where consistency and reproducibility are essential.


Autophagy detected in living cells

GFP compabtible Apoptosis detection

 

Enzyme Activity Assays

Screen Enzyme Modulators With Innovative Activity Assays

  • High purity, high activity control enzymes
  • Novel substrate/developer chemistry
  • Reduce interference from candidate compounds

Whether you are performing large-scale screens, or studying specific enzyme kinetics, our high activity enzymes, unique substrates, and convenient kits are designed to deliver high-quality hits.


Modified Substrate-Based Format

FRET-Based Format



Antibody-Based Format

 

Rapid Generation of Monoclonal Antibodies

A Novel Technology for mAb Detection and Isolation

The Direct Selection of Hybridomas (DiSH) kit is a novel system in which mAb-secreting hybridoma cells co-express significant amounts of the membrane form of the secreted immunoglobulin (Ig) on their surfaces. Subsequently, hybridomas with high-affinity for the target antigen are labeled. And once labeled, these hybridomas can be isolated from among a pool of hundreds of thousands of cells in a matter of hours using Fluorescence activated cell sorting (FACS) or magnetic separation. The total elapsed time from harvest to the first screening is typically 16 days, resulting in improved efficiency for detection of highly specific mAb.


Key Features of the Technology:

  • Decreased time and labor costs
  • Improved selection of specific, stable hybridomas
  • Minimal manipulation of cloned cell lines relative to approaches requiring Limiting Dilution Subcloning
 

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