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Histamine ELISA kit

Ultra-sensitive Histamine ELISA kit enabling detection of Histamine in serum, plasma, urine, and tissue culture media in just 2 hours.
 
ENZ-KIT140-0001 96 wells 505.00 USD
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  • Sensitive measurement of Histamine, detecting as little as 0.03 ng/ml
  • Unique assay, does not require an additional acylation step
  • Broad dynamic range suitable for a large variety of samples
  • High throughput format with results in 2 hours for up to 40 samples in duplicate
The Histamine ELISA kit is a colorimetric competitive immunoassay kit with results in 2 hours.
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Product Specification

Sensitivity:0.03 ng/ml (0.098-25 ng/ml)
 
Assay Time:2 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of histamine in tissue culture media, serum, plasma and urine in human, mouse, rat, and dog (serum only) samples.
 
Species reactivity:Human, Mouse, Rat
Dog
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter Plate, Assay Buffer 13, Standard, Wash Buffer Concentrate, Antibody, Tracer, Conjugate, Substrate, and Stop Solution
 
Scientific Background:Histamine is an endogenous short-acting biogenic amine synthesized from the amino acid histidine and is widely distributed throughout the body. Histamine is an important mediator of immediate-type-allergic reactions. Normally, there is a minimal amount of Histamine circulating in the human body. Elevations in plasma and tissue histamine levels have been reported during anaphylaxis and experimental systems investigating the allergic responses of the skin and airways. Because of its potent role in the immune response, histamine in the body is present in a very transitory state and can only be measured within minutes of release. The half-life of histamine in a biological system is four minutes before its conversion to n-methyl histamine. N-methyl histamine is a major product of histamine metabolism, is present in urine and has a longer half-life than that of histamine. This assay reacts equally to N-methyl histamine and histamine, making it an excellent choice for measuring histamine levels in urine.

Histamine is also in a class of neurotransmitters called “Small Molecule Neurotransmitter Substances”. This group includes molecules such as Serotonin, Epinephrine and Dopamine. Additionally, histamine plays a role in gastric acid secretion, assisting in the induction of acid production.
 

Product Literature References

IL-33 ameliorates Alzheimer's disease-like pathology and cognitive decline: A.K. Fu, et al.; PNAS 113, E2705 (2016), Application(s): Measured plasma histamine level, Abstract; Full Text

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Histamine Immunoassay kit
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