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Cell migration assay, flex kit

Reproducibly monitor real-time cell migration without the use of special instrumentation
 
ENZ-KIT117-0001 96 wells 335.00 USD
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  • Membrane-free, Cell Migration or Invasion: perform studies without manipulating transmembrane inserts - observe live images of cell movement
  • Creative Assay Design: coat any ECM or BME on the plate to create a 2-D environment for cell migration or cell invasion assays.
  • Preserve Cell Morphology: eliminate need for cells to penetrate through a polycarbonate membrane; cells can move across a treated surface or within a user-applied ECM
  • Real-time Monitoring: monitor changes in cell structure in real-time throughout the experiment.
  • Versatile Detection: analyze cells treated with multiple fluorescent probes, labels or stains by using a microscope, digital imaging system or fluorescence microplate reader.
  • Reproducible Results: obtain greater reproducibility using the Cell Seeding Stoppers compared to wound healing or scratch assays.
  • Flexible: perform kinetic or endpoint assays without the use of special instrumentation.
The Cell migration assay, flex kit is a reproducible, sensitive, and flexible assay that can be used to monitor cell migration or cell invasion. The Cell migration assay, flex kit now gives researchers more control over designing a cell migration assay. Each Cell migration assy, flex kit contains the Cell Seeding Stoppers for creating a detection zone at the center of each well in a 96-well plate. Since the stoppers are not pre-inserted into the wells, researchers can coat the plate with an extracellular matrix (ECM) component to design their assay. Researchers may also apply 3-dimensional overlays in each well to watch how cells invade and respond to various compounds (chemokinesis).

The Cell migration assay, flex kit is designed to be used with any stain or labeling technique. Readout can be performed by microscopy or use of a microplate reader.

The Cell migration assay, flex kit has been designed for use with adherent cell cultures. This assay has been successfully used with 3T3-Swiss albino, HT-1080, HCEC, HUVEC, and MCF10A cell lines.
ENZ-KIT117 assay formats
Fig 1. Formats of Cell migration assays.
ENZ-KIT117 schematic
Fig 2. Schematic of Cell migration assay, flex kit.
ENZ-KIT117 migration
Fig 3. Cell migration data using cell tracking stain.
Please mouse over
ENZ-KIT117 assay formats ENZ-KIT117 schematic ENZ-KIT117 migration

Product Specification

Applications:Fluorescence microscopy, Fluorescent detection
 
Application Notes:2-D assay for investigating cell migration of adherent cell lines
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Kit/Set Contains:Tissue Culture treated, stopper compatible 96-well plate (black, clear bottom)
Cell seeding stoppers (96)
Cell migration detection mask
Cell migration stopper tool
 

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