Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

AMP'D® HSP70 high sensitivity ELISA kit

Ultra-sensitive (7 pg/ml) AMP'D® HSP70 high sensitivity ELISA kit enabling the ability to use less sample and detect both baseline and upregulated levels of human, mouse and rat Hsp70 (Hsp72), a major chaperone, cancer biomarker, and key cell stress regulator.
 
ENZ-KIT-101-0001 96 wells 592.00 USD
Do you need bulk/larger quantities?
 
  • Ultra-sensitive measurement of Hsp70, detecting as little as 7 pg/ml
  • Ability to detect both baseline and upregulated/stressed levels of Hsp70 in human, mouse and rat serum and plasma samples
  • Negligible reactivity from similar Hsp70 family members (Hsc70/Hsp73, Grp78, DnaK, or Hsp71)
  • High throughput format with results in 4.5 hours for up to 38 samples in duplicate
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The AMP'D® HSP70 high sensitivity ELISA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4.5 hours.
ENZ-KIT-101 std curve
ENZ-KIT-101 parallelism
ENZ-KIT-101 kitbox
ENZ-KIT-101 plate
Please mouse over
ENZ-KIT-101 std curve ENZ-KIT-101 parallelism ENZ-KIT-101 kitbox ENZ-KIT-101 plate

Product Specification

Alternative Name:Heat shock protein 70, HSP72
 
Sensitivity:7 pg/ml (range 39-500 pg/ml)
 
Assay Time:4.5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of normal and upregulated (stressed) levels of HSP70 in serum and plasma of human, mouse, and rat origin.
 
Species reactivity:Human, Mouse, Rat
 
Crossreactivity:HSP72 (human) (100%), HSP70 (rat) (117.6%), HSP70B' (human) (5.4%), HSP70 (salmon) (0.8%), DnaK (E.coli) (0.5%), and <0.016%: Hsc70 (bovine), Grp78 (hamster), HSP71 (M. tuberculosis)
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter Plate, Assay Buffer 28, Standard, Wash Buffer Concentrate, Antibody, Conjugate, and AMP'D® (Signal Amplification) Substrate, Amplifier, Substrate Diluent, Amplifier Diluent, and Stop Solution
 
Scientific Background:The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal  pathway.
 
UniProt ID:P0DMV8 (HSPA1A), P0DMV9 (HSPA1B)
 
GenBank ID:M11717
 
Gene/Protein Identifier:NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM)
 

Product Literature References

A comparison of two commercially available ELISA methods for the quantification of human plasma heat shock protein 70 during rest and exercise stress: B.J. Lee, et al.; Cell Stress Chaperones 20, 917 (2015), Application(s): ELISA using human plasma, Abstract; Full Text

Related Products

HSP70 high sensitivity ELISA kit 

Only commercially available kit specifically validated and sensitive enough to quantify HSP70 (HSP72) in serum and plasma samples.
ELISA, Colorimetric detection | Print as PDF
 
ADI-EKS-715 96 wells 567.00 USD
Do you need bulk/larger quantities?
 

HSP70 ELISA kit 

Widely used ELISA HSP70 kit for chaperone and stress research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-EKS-700B 96 wells 562.00 USD
Do you need bulk/larger quantities?
 

Anti-HSP70 IgG/A/M (human), ELISA kit 

Most comprehensive portfolio of heat shock protein (chaperone) products commercially available.
ELISA, Colorimetric detection | Print as PDF
 
ADI-EKS-750 96 wells 630.00 USD
Do you need bulk/larger quantities?
 

HSP70B’ ELISA kit 

Only commercially available ELISA kit for ultra-sensitive HSP70B’ detection in cell lysates, serum, and tissue.
ELISA, Colorimetric detection | Print as PDF
 
ADI-EKS-725A 96 wells 725.00 USD
Do you need bulk/larger quantities?
 

Grp78/BiP ELISA kit 

High-sensitivity ELISA kit for quantifying both normal and upregulated levels of Grp78/BiP for unfolded protein response, cancer and neurodegenerative disease research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-214-0001 96 wells 541.00 USD
Do you need bulk/larger quantities?
 

AMP'D® ELISA Signal Amplification Kit 

AMP'D® ELISA Signal Amplification Kit provides up to 50-fold increase in sensitivity over traditional ELISAs while detecting lower concentrations of target in samples.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT-100-0001 96 wells 1 assay 82.00 USD
 
ENZ-KIT-100-0005 5x96 wells 5 assays 306.00 USD
Do you need bulk/larger quantities?
 

Goat anti-rabbit IgG, polyclonal antibody (AP conjugate) 

ELISA | Print as PDF
 
ENZ-ABS257-5000 5 ml 37.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
Assay Layout Sheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
HSP70
By biological activity:
HSP70 Immunoassay kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
Download as PDF

Catalogs
Immunoassay Kits
Immunoassay Kits
Download as PDF

Product Flyers
AMP’D® HSP70 High Sensitivity ELISA Kit
AMP’D® HSP70 High Sensitivity ELISA Kit
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,