- Innovative - only source for HSP70B’ ELISA, due to patent protection
- Fast - results from up to 40 samples in duplicate in less than 4.5 hours
- Quantitative - obtain statistically signifcant results
- Save - time and precious sample compared to Western blot analysis
The HSP70B’ EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 4.5 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
Product Details
| Alternative Name: | Heat shock protein 70, HSP72 |
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| Sensitivity: | 62 pg/ml (range 0.156 - 10 ng/ml) |
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| Assay Time: | <4.5 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of HSP70B' in cell lysates, serum, and tissue of human origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | Microtiter plate, 5X Extraction reagent, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent, HRP Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2 |
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| Scientific Background: | The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. |
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| UniProt ID: | P17066 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation: N. Mirza, et al.; Transpl. Immunol.
36, 25 (2016),
Application(s): Heat shock protein detection,
Abstract;
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