Sensitive measurement of HSP70, measuring as low as 200 pg/ml
Fully quantitative results compared to Western blot analysis
Highly specific, with minimal cross-reactivity with other HSP
Validated in stressed and non-stressed samples
The HSP70 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4.5 hours. Absorbance is read at 450 nm. This kit is ideal for measuring intracellular HSP70 (HSP72). Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
Product Details
Alternative Name:
Heat shock protein 70, HSP72
Sensitivity:
200 pg/ml (range 780 - 50,000 pg/ml)
Assay Time:
4.5 hours
Applications:
ELISA, Colorimetric detection
Application Notes:
For the quantitative determination of HSP70 in cell lysates and tissue of human, mouse, and rat origin. Prod. No. ADI-EKS-715 is recommended for the detection of Hsp70 in serum and plasma samples.
Wavelength:
450 nm
Species reactivity:
Human, Mouse, Rat
Use/Stability:
Store all components at 4°C, except standard at -20°C.
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Shear stress induced by acute heat exposure is not obligatory to protect against endothelial ischemia-reperfusion injury in humans: H.W. Hemingway, et al.; J. Appl. Physiol. 132, 199 (2022), Abstract;
Ameliorating cancer cachexia by inhibiting cancer cell release of Hsp70 and Hsp90 with omeprazole: Z. Liu, et al.; J. Cachexia Sarcopenia Muscle 13, 636 (2021), Abstract;
HSP70, a Novel Regulatory Molecule in B Cell-Mediated Suppression of Autoimmune Diseases: L. Wang, et al.; J. Mol. Biol. 433, 166634 (2021), Application(s): PBMC culture supernatant, Abstract;
Mitigating multiple stresses in Pangasianodon hypophthalmus with a novel dietary mixture of selenium nanoparticles and Omega-3-fatty acid: N. Kumar, et al.; Sci. Rep. 11, 19429 (2021), Abstract;
Nicotinamide and heat preconditioning–Effects on hepatic HSP70, carbohydrate and oxidative disturbances in STZ-induced diabetic rats: K. Gerazova-Efremova, et al.; J. Therm. Biol. 91, 102645 (2020), Application(s): ELISA using rat tissue, Abstract;
Physiological stress does not increase with urbanization in European blackbirds: Evidence from hormonal, immunological and cellular indicators: J.D. Ibanez-Alamo, et al.; Sci. Total Environ. 721, 137332 (2020), Application(s): ELISA using bird red blood cells, Abstract;
In Vivo Comparative Study on Acute and Sub-acute Biological Effects Induced by Ultrafine Particles of Different Anthropogenic Sources in BALB/c Mice: F. Farina, et al.; Int. J. Mol. Sci. 20, 2805 (2019), Application(s): Cell culture supernatants, Abstract; Full Text
The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats: D. Stygar, et al.; Cell. Stress Chaperones 24, 427 (2019), Application(s): ELISA using rat tissue, Abstract; Full Text
Understanding how dogs age: longitudinal analysis of markers of inflammation, immune function, and oxidative stress: J.E. Alexander, et al.; J. Gerontol. A Biol. Sci. Med. Sci. 73, 720 (2018), Application(s): ELISA using dog PBMCs, Abstract;
Secretion of cytokines and heat shock protein (HspA1A) by ovarian cancer cells depending on the tumor type and stage of disease: M. Nowak, et al.; Cytokine 89, 136 (2017), Application(s): HSP70 in ovarian cancer cells, Abstract;
Diagnostic Role for Antibodies against Heat Sock Protein 70 In Perilymphatic Fluid of Children with Cochlear Implant Surgery: A Case Control Study in Tehran, Iran: M.R. Shokrollahi, et al.; Int. J. Curr. Res. Aca. Rev. 4, 184 (2016), Application(s): HSP70 levels measured in blood samples , Full Text
Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping: N. Frara, et al.; BMC Musculoskelet. Dis. 17, 34 (2016), Application(s): HSP72 measured in tissue lysates, Abstract; Full Text
Heat shock protein–based therapy as a potential candidate for treating the sphingolipidoses: T. Kirkegaard, et al.; Sci. Transl. Med. 8, 355 (2016), Application(s): ELISA using mouse tissue, Abstract; Full Text
Linking HSP90 target occupancy to HSP70 induction and efficacy in mouse brain: K. Thirstrup, et al.; Pharmacol. Res. 104, 197 (2016), Application(s): ELISA using mouse cell and tissue lysates, Abstract;
Myokines In Response To A Tournament Season Among Young Tennis Players: K. Witek, et al.; Biomed Res. Int. 2016, Article ID 1460892 (2016), Application(s): Blood sampling and cytokine analysis, serum HSP analysis, Abstract; Full Text
Physiological, Morphological and Behavioural Responses of Self-Feeding Precocial Chicks Copying with Contrasting Levels of Water Salinity during Development: A.R. Rocha, et al.; PLoS One 11, e0165364 (2016), Application(s): Hsp70 concentration fromthe erythrocyte lysates , Abstract; Full Text
Transgenic sickle cell trait mice do not exhibit abnormal thermoregulatory and stress responses to heat shock exposure: Y. Chen & A. Islam; Blood Cell. Mol. Dis. 59, 124 (2016), Application(s): Mouse tissue homogenates from liver, heart and gastrocnemius, Abstract;
A first-in-human phase I, dose-escalation, multicentre study of HSP990 administered orally in adult patients with advanced solid malignancies: A. Spreafico, et al.; Br. J. Cancer 112, 650 (2015), Abstract; Full Text
Antioxidant Reviving Role of Mint-Tea on Placenta During Gestational Diabetes Mellitus: P. Ekambaram, et al.; International Journal of Science and Research 4, 501 (2015), Application(s): ELISA using human GDM (Gestational diabetes mellitus) placental tissue homogenate, Full Text
Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment: L. Shervington, et al.; J. Cancer 6, 786 (2015), Application(s): ELISA using human cell lysate, Abstract; Full Text
Effect of copper nanoparticles and copper sulphate on oxidation stress, cell apoptosis and immune responses in the intestines of juvenile Epinephelus coioides: W. Tao, et al.; Fish Shellfish Immunol. 44, 674 (2015), Application(s): ELISA, Abstract;
Effect Of Early Heat Acclimation On Immunity And Some Intestinal Functions And Heat Shock Protein Post-Weaned Rabbits During Summer Season: A.S. O EI-Badry, et al.; Egypt. Poult. Sci. 35, 123 (2015), Application(s): ELISA using rabbit small intestine cell supernatant, Full Text
Increased plasma levels of heat shock protein 70 associated with subsequent clinical conversion to mild cognitive impairment in cognitively healthy elderly: S.J. Son, et al.; PLoS One 10, e0119180 (2015), Application(s): ELISA using human plasma, Abstract; Full Text
Lazaroids U83836E and U74389G are potent, time-dependent inhibitors of caspase-1: M. Kawarski, et al.; Chem. Biol. Drug Des. 86, 1049 (2015), Application(s): Analysis of tissue lysates, Abstract; Full Text
Repeated muscle damage blunts the increase in heat strain during subsequent exercise heat stress: A. Dolci, et al.; Eur. J. Appl. Physiol. 115, 1577 (2015), Abstract;
Role of Hsp70 and the Associated Signaling Molecules in Fish Adipocytes during Pollutants Induced Hypoxia: P. Ekambaram, et al.; IOSR J. Environ. Sci. Toxicol. Food Technol. 9, 16 (2015), Application(s): ELISA using fish adipocytes, Full Text
Adrenergic and glucocorticoid modulation of the sterile inflammatory response: S. Cox, et al.; Brain Behav. Immun. 36, 183 (2014), Application(s): EIA using human plasma samples, Abstract;
Chronic treatment with 17-DMAG improves balance and coordination in a new mouse model of Machado-Joseph disease: A. Silva-Fernandes, et al.; Neurotherapeutics 11, 433 (2014), Application(s): ELISA using mouse tissue, Abstract; Full Text
Contesting the dogma of an age-related heat shock response impairment: implications for cardiac-specific age-related disorders: A. Carnemolla, et al.; Hum. Mol. Genet. 23, 3641 (2014), Application(s): ELISA using mouse tissue, Abstract; Full Text
Early Response Roles for Prolactin Cortisol and Circulating and Cellular Levels of Heat Shock Proteins 72 and 90a in Severe Sepsis and SIRS: K. Vardas, et al.; BioMed Research International 2014, 803561 (2014), Application(s): Human plasma HSP70 levels, Abstract; Full Text
Each to their own: skeletal muscles of different function use different biochemical strategies during aestivation at high temperature: K.M. Young, et al.; J. Exp. Biol. 216, 1012 (2013), Application(s): ELISA using frog tissue, Abstract;
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): EIA using mouse tissue extract, Abstract; Full Text
Impact of seasonal variation on HSP70 expression quantitated in stressed fish hepatocytes: E. Padmini, et al. ; Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 151, 278 (2008), Application(s): EIA using fish cell lysates, Abstract;
The effect of the rate of heat storage on serum heat shock protein 72 in humans: F. Amorim, et al. ; Eur. J. Appl. Physiol. 104, 965 (2008), Application(s): EIA using human serum, Abstract;
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R844 (2007), Application(s): EIA using human vastus lateralis muscle tissue, Abstract; Full Text
Ultra-sensitive (7 pg/ml) AMP'D® HSP70 high sensitivity ELISA kit enabling the ability to use less sample and detect both baseline and upregulated levels of human, mouse and rat Hsp70 (Hsp72), a major chaperone, cancer biomarker, and key cell stress regulator.
High-sensitivity ELISA kit for quantifying both normal and upregulated levels of Grp78/BiP for unfolded protein response, cancer and neurodegenerative disease research.
