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AbeoClone™ (Base Semi-solid Medium) (2X)

Semi-solid Base media optimized for selection of hybridoma colonies and monoclonal cell line isolation.
 
ENZ-70009-0045 45 ml 152.00 USD
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  • Compared with limited dilution cloning, semi-solid cloning is faster and requires fewer resources to isolate monoclonal cell lines
  • Enables isolation of diverse clones with a variety of growth rates and productivities compared to selection in bulk liquid medium
  • Individual cells are physically separated by suspension in the viscous medium and are able to grow into discrete colonies that can be easily isolated
  • Rare, high-producing clones are easily isolated compared with selection in liquid medium
AbeoClone™ (Base Semi-solid Medium) is a methylcellulose medium that is combined with a 2X concentrate of the user’s own liquid medium to create a custom semi-solid cloning medium. The medium is recommended for the cloning of a variety of mammalian cell lines including suspension-adapted lines such as CHO-S and hybridomas, in addition to some cell lines that grow adherently.  The medium is chemically defined, protein-free and animal component-free and formulated without L-glutamine or selection agents. This medium is compatible with dihydrofolate reductase (DHFR), glutamine synthase (GS) and hypoxanthine-aminopterin-thymidine (HAT) systems. In this semi-solid matrix, colonies are easily manually harvested using a sterile micropipette tip or with automated colony-picking systems. 

Product Specification

Alternative Name:Hybridoma Dish™ Kit semi-solid medium
 
Application Notes:Uses:
Cell line development
Custom media
Hybridoma generation
Monoclonal cell line isolation and development
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. Storage at 2-8°C is not recommended.
 

Product Literature References

Engineered cell surface expression of membrane immunoglobulin as a means to identify monoclonal antibody-secreting hybridomas: P.W. Price, et al.; J. Immunol. Methods 343, 28 (2009), Application(s): Hyridoma screening, Abstract; Full Text

General Literature References

Antibody production by hybridomas: J.W. Goding; J. Immunol. Methods 39, 285 (1980), Abstract;
Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies: J. Sharon, et al.; PNAS 76, 1420 (1979), Abstract; Full Text
Continuous culture of fused cells secreting antibody of predefined specificity: G. Köhler & C. Milstein; Nature 256, 495 (1975), Abstract; Full Text

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