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MM 1-43

Neuronal detection
 
ENZ-52251 1 mg 281.00 USD
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MM 1-43 is a cationic dye, equivalent to membrane probe FM® 1-43. MM 1-43 is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The staining of cell-surface specific membranes is useful for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. Wavelength Maxima: Excitation 510nm, Emission 626nm

Product Specification

Alternative Name:N-(3-Triethylammoniumpropyl)-4-(4-(dibutylamino)styryl)pyridinium dibromide
 
Formula:C30H49Br2N3
 
MW:611.5
 
CAS:149838-22-2
 
Purity:≥90% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells: D.R. Williams, et al.; Nat. Protoc. 3, 835 (2008), Abstract;
FM 1-43 labeling of synaptic vesicle pools at the Drosophila neuromuscular junction: P. Verstreken, et al.; Methods Mol. Biol. 440, 349 (2008), Abstract;

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