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PATHO-GENE® HRP-AEC HPV in situ typing assay (types 6/11, 16/18 and 31/33/51)

 
ENZ-32877 20 assays 2,550.00 USD
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The PATHO-GENE® HRP-AEC Human papillomavirus (HPV) in situ typing assay for tissue sections provides reagents and materials for (a) preparation and pretreatment and (b) hybridization/detection and typing of HPV DNA (using Horseradish Peroxidase-AEC for signal generation) in 20 tissue biopsy specimens. This product is for research use only and is not to be used for diagnostic procedures.

The PATHO-GENE® HRP-AEC Human papillomavirus (HPV) in situ typing assay for tissue sections employs separate mixtures of HPV-specific probes to identify HPV types 6/11, 16/18, or 31/33/51. HPV types 6 and 11 are primarily associated with benign lesions presenting as condylomas and mild dysplasia. HPV types 16 and 18 are more commonly associated with cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS). HPV types 31, 33 or 51 can be associated with either condyloma or CIN and CIS, presumably dependent upon other, as yet undefined, contributing factors or agents. The PATHO-GENE® HRP-AEC Human papillomavirus (HPV) in situ typing assay for tissue sections can be used to detect and identify HPV DNA in tissue sections under conditions that preserve cellular morphology.

Product Specification

Applications:Colorimetric detection
 
Use/Stability:Store the Blue Counterstain at room temperature and protect from light.
 
Handling:Store the AEC Reagent at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:HPV 6/11 Probe Reagent, 1 ml (pink) Mixture of biotin-labeled HPV 6 and HPV 11-specific probes in buffered formamide and hybridization enhancers
HPV 16/18 Probe Reagent, 1 ml (purple) Mixture of biotin-labeled HPV 16 and HPV 18-specific probes in buffered formamide and hybridization enhancers
HPV 31/33/51 Probe Reagent, 1 ml (green) Mixture of biotin-labeled HPV 31, HPV 33 and HPV 51-specific probes in buffered formamide and hybridization enhancers
Post Hybridization Reagent, 30 ml (white) Buffered formamide
Detection Reagent, 30 ml (yellow) Streptavidin-biotinylated horseradish peroxidase in buffered saline and stabilizers and preservative
AEC Reagent, 1 ml (red) Aminoethylcarbazole in solvent
Reaction Buffer/Substrate Reagent, 5 x 6 ml (gray) Hydrogen peroxide in acetate buffer with stabilizer and preservative
Blue Counterstain, 30 ml (blue) Ready-to-use aqueous-based hematoxylin
Proteinase K, 2 x 5 mg Lyophilized powder
Quench Reagent, 30 ml (orange) 3% Hydrogen Peroxide in buffered saline
Wash Buffer Salts, 5 packets Each packet yields 1 liter of buffered saline
Pretreated Specimen Slides, 40 slides 20 single-well plus 20 double-well slides for mounting formalin-fixed paraffin-embedded tissue specimens

SOLD SEPARATELY: HPV 16 Probe Control Slide (Prod. No. ENZ-31877), Double-well slide with fixed HPV 16-positive cells

 
Technical Info/Product Notes:This product or the use of this product is covered by one or more Enzo patents, including, but not limited to, the following: U.S. Patent No. 4,994,373 and Canadian Patent No. 1,309,672; and patents pending.
 

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PATHO-GENE® AP-NBT/BCIP HPV in situ typing assay (types 6/11, 16/18 and 31/33/51) 

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For Research Use Only. Not for use in diagnostic procedures.
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