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Caspase-3 (human), (recombinant)

 
BML-SE169-5000 5000 U 404.00 USD
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Product Specification

Alternative Name:CPP32, Yama, Apopain
 
MW:17 + 12 kDa
 
Source:Produced in E. coli. Caspase-3 (CPP32; Yama; apopain) from human cDNA. The enzyme was cleaved and activated from the proenzyme
 
UniProt ID:P42574
 
Concentration:100 U/µl
 
Formulation:Liquid. In 50mM HEPES, pH 7.4, 100mM sodium chloride, 0.5% CHAPS, 1mM EDTA, 10% glycerol and 10mM dithiothreitol.
 
Purity:≥95% (SDS-PAGE)
 
Specific Activity:One U=1 pmol/min using Ac-DEVD-pNA (200 µM; Prod. No. ALX-260-033) as substrate at 30°C
 
Application Notes:Can be used to study enzyme regulation and kinetics, cleave target substrates and screen for inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The major effector caspase of apoptosis, caspase-3 is responsible for cleavage of multiple cellular targets.
 

Product Literature References

Approaches to design non-covalent inhibitors for human granzyme B (hGrB): M.S. Kim, et al.; Org. Biomol. Chem. 12, 8952 (2014), Abstract;
Propeptide-mediated inhibition of cognate gingipain proteinases: N.L. Huq, et al.; PLoS One 8, e65447 (2013), Abstract;
Vulnerable windows for developmental ethanol toxicity in the Japanese medaka fish (Oryzias latipes): S.L. Oxendine, et al.; Aquat. Toxicol. 80, 396 (2006), Abstract;
Protein kinase C delta blocks immediate-early gene expression in senescent cells by inactivating serum response factor: K. Wheaton, et al.; Mol. Cell Biol. 24, 7298 (2004), Abstract; Full Text
Cross-talk between calpain and caspase proteolytic systems during neuronal apoptosis: R.W. Neumar, et al.; J. Biol. Chem. 278, 14162 (2003), Abstract; Full Text
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N. A. Thornberry et al.; J. Biol. Chem. 272, 17907 (1997), Abstract;
Structure of recombinant human CPP32 in complex with the tetrapeptide acetyl-Asp-Val-Ala-Asp fluoromethyl ketone: P. R. E. Mittl et al.; J. Biol. Chem. 272, 6539 (1997), Abstract;
Substrate specificities of caspase family proteases: R. V. Talanian et al.; J. Biol. Chem. 272, 9677 (1997), Abstract;

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For Research Use Only. Not for use in diagnostic procedures.
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