Replaces Prod. #: BML-P412
Colorimetric substrate for caspase-3 (CPP32) (Km=9.7µM) and related cysteine proteases. Sequence is based on PARP (poly(ADPribose) polymerase) cleavage site Asp216 for caspase-3. Cleavage is monitored colorimetrically at 405nm.
Product Details
| Alternative Name: | Caspase-3 substrate (chromogenic) |
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| Sequence: | Ac-Asp-Glu-Val-Asp-pNA (pNA = p-Nitroaniline) |
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| Formula: | C26H34N6O13 |
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| MW: | 638.6 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥97% (HPLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO or buffer, pH 7. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Always use fresh solutions. |
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| Handling: | Keep cool and dry. |
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| Technical Info/Product Notes: | λmax of pNA is 400nm. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Self-assembly of nanomicelles with rationally designed multifunctional building blocks for synergistic chemo-photodynamic therapy: G. Gong, et al.; Theranostics
12, 2028 (2022),
Abstract;
Modulation of Apoptotic Cell Death and Neuroprotective Effects of Glutathione—L-Dopa Codrug Against H2O2-Induced Cellular Toxicity: S. Franceschellim, et al.; Antioxidants (Basel)
8, 319 (2019),
Abstract;
Full Text
The preclinical analysis of TW-37 as a potential anti-colorectal cancer cell agent: S. Lei, et al.; PLoS One
12, e0184501 (2017),
Abstract;
Full Text
Estrogen Receptor β Upregulates IGF1R Expression and Activity to Inhibit Apoptosis and Increase Growth of Medulloblastoma: C. J. Cookman, et al.; Endocrinology
156, 2395 (2015),
Application(s): Functional Studies,
Abstract;
Full Text
Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta et al.; J. Biol. Chem.
272, 1965 (1997),
Abstract;
Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem.
272, 9677 (1997),
Abstract;
Full Text
Activation of the CPP32 protease in apoptosis induced by 1-beta-D- arabinofuranosylcytosine and other DNA-damaging agents: R. Datta, et al.; Blood
88, 1936 (1996),
Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature
376, 37 (1995),
Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik et al.; Nature
371, 346 (1994),
Abstract;
Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol.
244, 615 (1994),
Abstract;
