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Caspase substrate
ALX-260-031-M001 1 mg 73.00 USD
ALX-260-031-M005 5 mg 204.00 USD
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Replaces Prod. #: BML-P411

Fluorogenic substrate for caspase-3 with Km=9.7µM. Also a substrate for caspase-1, -4, -7 and -8. The peptide sequence is based on the PARP cleavage site Asp216 for caspase-3. Ex.: 340-360nm, Em.: 440-460nm

Product Details

Alternative Name:Caspase-3 substrate (fluorogenic)
Sequence:Ac-Asp-Glu-Val-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)
Purity:≥97% (HPLC)
Solubility:Soluble in DMSO; dilute with buffer, pH 7.5.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Handling:Protect from light.
Technical Info/Product Notes:AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.
Regulatory Status:RUO - Research Use Only

Product Literature References

Bioluminescent RIPoptosome Assay for FADD/RIPK1 Interaction Based on Split Luciferase Assay in a Human Neuroblastoma Cell Line SH-SY5Y: P. Ghanavatian, et al.; Biosensors 13, 297 (2023), Abstract;
Characterization of new highly selective pyrazolo[4,3-d]pyrimidine inhibitor of CDK7: M. Kovalová, et al.; Biomed. Pharmacother. 161, 114492 (2023), Abstract;
Cardioprotective Effects of Oroxylum indicum Extract Against Doxorubicin and Cyclophosphamide-Induced Cardiotoxicity: S.R. Pondugula, et al.; Cardiovasc. Toxicol. 22, 67 (2022), Abstract;
Design, Synthesis, In Silico Studies and Inhibitory Activity towards Bcr-Abl, BTK and FLT3-ITD of New 2,6,9-Trisubstituted Purine Derivatives as Potential Agents for the Treatment of Leukaemia: J. Bertrand, et al.; Pharmaceutics 14, 1294 (2022), Abstract;
Disulfiram oxy-derivatives induce entosis or paraptosis-like death in breast cancer MCF-7 cells depending on the duration of treatment: M. Solovieva , et al.; Biochim. Biophys. Acta Gen. Subj. 1866, 130184 (2022), Abstract;
Aromatic monophenols from cinnamon bark act as proteasome inhibitors by upregulating ER stress, suppressing FoxM1 expression, and inducing apoptosis in prostate cancer cells: S. Gopalakrishnan, et al.; Phytother. Res. 35, 5781 (2021), Abstract;
Exogenous Introduction of Initiator and Executioner Caspases Results in Different Apoptotic Outcomes: F. Anson, et al.; JACS Au 1, 1240 (2021), Abstract;
β-hydroxybutyric acid attenuates oxidative stress and improves markers of mitochondrial function in the HT-22 hippocampal cell line: M. Majrashi, et al.; J. Integr. Neurosci. 20, 321 (2021), Abstract;
Early-onset impairment of the ubiquitin-proteasome system in dopaminergic neurons caused by α-synuclein: C. McKinnon, et al.; Acta Neuropathol. Commun. 8, 17 (2020), Abstract; Full Text
Neuroprotective Effects of Methyl Caffeate against Hydrogen Peroxide-Induced Cell Damage: Involvement of Caspase 3 and Cathepsin D Inhibition: D. Jantas, et al.; Biomolecules 10, 1530 (2020), Abstract; Full Text
Vitamin B12b Enhances the Cytotoxicity of Diethyldithiocarbamate in a Synergistic Manner, Inducing the Paraptosis-Like Death of Human Larynx Carcinoma Cells: M. Solovieva, et al.; Biomolecules 10, 69 (2020), Abstract;
C33-A cells transfected with E6*I or E6*II the short forms of HPV-16 E6, displayed opposite effects on cisplatin-induced apoptosis: C.E. Vaisman, et al.; Virus Res. 247, 94 (2018), Abstract;
Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells: S. Zielke, et al.; Cell Death Dis. 9, 994 (2018), Abstract; Full Text
Caspase-6 Undergoes a Distinct Helix-Strand Interconversion upon Substrate Binding: K.B. Dagbay, et al.; J. Biol. Chem. 292, 4885 (2017), Abstract; Full Text
Study of commonly used organophosphate pesticides that induced oxidative stress and apoptosis in peripheral blood lymphocytes of rats: A. Ojha, et al.; Hum. Exp. Toxicol. 36, 1158 (2017), Abstract;
The effect of ARC ablation on skeletal muscle morphology, function, and apoptotic signaling during aging: K. Vorobej, et al.; Exp. Gerontol. 101, 69 (2017), Abstract;
Cross-talk between two antioxidants, thioredoxin reductase and heme oxygenase-1, and therapeutic implications for multiple myeloma: P.V. Raninga, et al.; Redox Biol. 8, 175 (2016), Application(s): Caspase-3 activity assay, Abstract;
Data on skeletal muscle apoptosis, autophagy, and morphology in mice treated with doxorubicin: T.L. Campbell, et al.; Data Brief 7, 786 (2016), Application(s): Assessed caspase-3, -8, and -9 activity in mixed gastrocnemius homogenates, Abstract;
Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts: R. Ghosh, et al.; Int. J. Cardiol. 223, 923 (2016), Application(s): Measurement of Caspase 3 activity - mouse heart, liver, and kidney, Abstract;
Effect of mitochondrial fission inhibition on C2C12 differentiation: D. Bloemberg, et al.; Data Brief 7, 634 (2016), Application(s): Caspase activity, Abstract; Full Text
Efficient Activation of Apoptotic Signaling during Mitotic Arrest with AK301: A. Chopra, et al.; PLoS One. 11, e0153818 (2016), Application(s): Caspase-3 assay, Abstract; Full Text
Enhanced apoptotic cancer cell killing after Foscan photodynamic therapy combined with fenretinide via de novo sphingolipid biosynthesis pathway: N.B. Boppana, et al.; J. Photochem. Photobiol. B 159, 191 (2016), Application(s): DEVDase activity assay, Abstract;
Increased apoptosis and browning of TAK1-deficient adipocytes protects against obesity: A. Sassmann-Schweda, et al.; JCI Insight 1, e81175 (2016), Application(s): Caspase-3 activity assay, Abstract; Full Text
Induction of cell death by the novel proteasome inhibitor marizomib in glioblastoma in vitro and in vivo: C.A. Manton, et al.; Sci. Rep. 25, 6 (2016), Application(s): Caspase 3/7 activity assay, Abstract; Full Text
Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway: M.Y. Cherepkova, et al.; Cell Death Dis. 14, e2050 (2016), Application(s): Measured caspase-3 activity in vitro, Abstract;
Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro: F. Brisdelli, et al.; Life Sci. 158, 89 (2016), Application(s): Caspase activity, Abstract;
The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila: L. Koerver, et al.; Cell Death Differ. 23, 2019 (2016), Application(s): DEVDase assay from testes lysate, Abstract;
Treatment of Mycobacterium tuberculosis-Infected Macrophages with Poly(Lactic-Co-Glycolic Acid) Microparticles Drives NFκB and Autophagy Dependent Bacillary Killing: C. Lawlor, et al.; PLoS One 11, e0149167 (2016), Application(s): Caspase activity assays, Abstract; Full Text
Accentuating Death Signaling in Colon Cancer Cells: A Chemical Biology Approach: A. Chopra; (2015), (PhD thesis), Application(s): Caspase-3 assay, Full Text
Assessment of sequential combination of 5-Fluorouracil-loaded-chitosan-nanoparticles and ALA-Photodynamic therapy on HeLa cell line: M. Benito-Miguel, et al.; Photodiagnosis Photodyn. Ther. 12, 466 (2015), Application(s): Assay using HeLa cells, Abstract;
Calpain-3 impairs cell proliferation and stimulates oxidative stress-mediated cell death in melanoma cells: D. Moretti, et al.; PLoS One 10, e0117258 (2015), Application(s): Fluorometric Assay, Abstract; Full Text
Efficacy of panobinostat and marizomib in acute myeloid leukemia and bortezomib-resistant models: F.F. Corrales-Medina, et al.; Leuk. Res. 39, 371 (2015), Application(s): Western Blotting, Abstract;
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype: V.A. Fajardo, et al.; Dis. Model Mech. 8, 999 (2015), Application(s): Fluorescence, Abstract; Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 300, R531 (2011), Abstract; Full Text
Purification and catalytic properties of human caspase family members: M. Garcia-Calvo, et al.; Cell Death Differ. 6, 362 (1999), Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik et al.; Nature 371, 346 (1994), Abstract;