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Dimethyl-lysine polyclonal antibody (affinity purified)

 
BML-SA668-0050 50 µg 378.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Dimethylated KLH.
 
Species reactivity:Species independent
 
Specificity:Recognizes proteins containing dimethylated lysine residues in SDS-PAGE immunoblots.
 
Crossreactivity:Does not cross-react with monomethylated or trimethylated lysines in peptides.
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (0.02-0.2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Affinity-purified.
 
Formulation:Liquid. In PBS, pH 7.2.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
bml-sa668

Figure 1: Detection of dimethyl lysine using PAb to Dimethyl-lysine (Affinity Purified).Method: 20 µg of HeLa cell nuclear extract was subjected to SDS-PAGE and then transferred to nitrocellose. The blot was probed with PAb to Dimethyl-lysine (Affinity Purified) (0.2 µg/ ml) and developed colorimetrically.

Lane 1: PAb to Dimethyl-lysine (Affinity Purified) alone. Lane 2: PAb to Dimethyl-lysine (Affinity Purified) preincubated with dimethylated BSA. Lane 3: PAb to Dimethyl-lysine (Affinity Purified) preincubated with untreated BSA.

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Lysine
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Lysine Polyclonal antibody
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