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Melanocortin receptor 3 (extracellular) polyclonal antibody

 
BML-SA640-0050 50 µl 345.00 USD
 
BML-SA640-0200 200 µl 517.00 USD
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Product Specification

Alternative Name:MC3R
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 102-117 of rat MC3R (melanocortin receptor 3).
 
UniProt ID:P32244
 
Species reactivity:Human, Mouse, Rat
 
Applications:Flow Cytometry, IHC, WB
 
Blocking Peptide:Included: Melanocortin Receptor 3 Control Peptide (40µg). Supplied lyophilized.
 
Purity Detail:Affinity purified IgG.
 
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide.
 
Reconstitution:To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. To lyophilized peptide, add 100µl of deionized water.
 
Use/Stability:Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The melanocortin receptors are members of the G protein-coupled receptor superfamily. Their ligands, the melanocortins, are peptides consisting of µ-, β- and γ-melanocyte stimulating hormone and adrenocorticotropic hormone. MC3R binds all melanocortins with equal affinity. Agouti-related protein (AGRP) is the high affinity endogenous antagonist of MC3R. It is expressed in the brain, placenta, and gut tissues, but not in the adrenal cortex or melanocytes.
 
Technical Info/Product Notes:PREADSORPTION CONTROL: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium aide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.
 
BML-SA640 WB
Figure: Western blot analysis of mouse (lanes 1, 3) and rat (lanes 2, 4) brain lysates: Lane 1, 2: Anti-MC3R (extracellular). Lanes 3, 4: Anti-MC3R (extracellular) preincubated with the control peptide
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BML-SA640 WB

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