Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Proteasome 20S β5i subunit (human) polyclonal antibody

 
BML-PW8355-0025 25 µl 118.00 USD
 
BML-PW8355-0100 100 µl 356.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-210-326

Product Specification

Alternative Name:Proteasome subunit β type-8, LMP7, RING10 protein, Macropain subunit C13
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 262-276 (T262DVSDLLHQYREANQ276) of human proteasome 20S (β5i subunit).
 
UniProt ID:P28062
 
Gene/Protein Identifier:PSMB8 (gene name), 5696 (Entrez Gene ID)
 
Species reactivity:Human
 
Specificity:Recognizes the β5i subunit of the 20S proteasome.
 
Applications:IHC, WB
 
Application Notes:Detects bands of ~25kDa (mature proteasome 20S β5i subunit) and of ~29kDa (precursor) by Western blot.
 
Formulation:Liquid. Antiserum containing 10mM sodium azide.
 
Use/Stability:Dilute with PBS, pH 7.2-7.4 containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C (do not freeze) and use within 1 month.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles.  The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome.  Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle.  Each subunit is located in a unique position within the α- or β-rings. Lmp2, Lmp7 and MECL are interferon gamma-inducible catalytic subunits of the 20S proteasome which may replace the constitutive catalytic subunits, delta, X and Z respectively, during proteasome biogenesis.  Lmp2 and Lmp7 alter the cleavage site specificity of the 20S proteasome and are required for the efficient generation of T cell epitopes from a number of viral proteins and for optimal MHC class I cell surface expression.
 
Technical Info/Product Notes:Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
 
bml-pw8355 wb
Figure: Western blot analysis of HeLa cell lysate with and without prior stimulation with interferon γ using Proteasome 20S beta5i subunit (human), pAb (Prod. No. BML-PW8355).Method: SDS-PAGE was followed by blotting onto PVDF and probing with antibody BML-PW8355 and others. Antibody dilution 1:10’000.
Please mouse over
bml-pw8355 wb

Product Literature References

Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Western Blot, Abstract;

General Literature References

Dendritic cells up-regulate immunoproteasomes and the proteasome regulator PA28 during maturation: A. Macagno, et al.; Eur. J. Immunol. 29, 4037 (1999), Abstract;
Immunoproteasome assembly: cooperative incorporation of interferon gamma (IFN-gamma)-inducible subunits: T.A. Griffin, et al.; J. Exp. Med. 187, 97 (1998), Abstract; Full Text
A third interferon-gamma-induced subunit exchange in the 20S proteasome: M. Groettrup, et al.; Eur. J. Immunol. 26, 863 (1996), Abstract;

Related Products

Proteasome 20S (beta subunits) antibody sampler pack 

Print as PDF
 
BML-PW8905-0001 12x10 µl 704.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Proteasome 20S
By biological activity:
Proteasome 20S Polyclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,