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Carboxyfluorescein multi-caspase activity kit

 
BML-AK117-0001 1 Kit 508.00 USD
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The Carboxyfluorescein Multi-Caspase Activity Kit detects apoptosis through the covalent binding of a green fluorescent probe to active caspase enzymes in living cells. The carboxyfluorescein-labeled caspase inhibitor probe (FAM-VAD-FMK; excitation at 490 nm and emission at 520 nm), comprising a carboxyfluorescein derivative of valylalanylaspartic acid fluoromethyl ketone (VAD-FMK), covalently binds to the reactive cysteine (Cys 285) on the large subunit of active caspase heterodimers. This probe is both cell permeable and non-cytotoxic during the time of the labeling procedure and allows detection of apoptosis by fluorescence microscopy, flow cytometry, and 96-well-plate based fluorometry. Use of the fluorescence microscope provides a qualitative view of the level of apoptosis induced as compared to the use of a fluorometer or a flow cytometer which will generate quantitative data. The Sulforhodamine Multi-Caspase Activity Kit for Apoptosis Detection is a related kit that contains a sulforhodamine-labeled probe (Ex: 550, Em: 595).

Product Specification

Applications:Fluorescent detection, HTS
Activity assay
 
Handling:Store the unopened kit (and each unopened component) at 2-8°C until the expiration date. Protect the FAM-VAD-FMK reagent from light at all times. Once reconstituted, the 150X FAM-VAD-FMK stock should be stored at -20°C protected from light. This reagent is stable for up to 6 months and may be thawed twice during that time.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:FAM-VAD-FMK Reagent, 10X Wash Buffer, Fixative, Propidium Iodide.
 

Product Literature References

Mcl-1 is a key regulator of the ovarian reserve: S. Omari, et al.; Cell. Death Dis. 6, e1755 (2015), Application(s): Assay using mouse oocytes, Abstract; Full Text

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