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Caspase-9 fluorometric assay kit

ALX-850-224-KI01 25 tests 156.00 USD
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Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate LEHD-AFC.

Product Specification

Alternative Name:ICE-LAP-6, Mch6
Applications:Fluorescent detection
Activity assay
Handling:Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening. Protect from light.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Contents:25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl LEHD-AFC Substrate (1mM), 100µl DTT (1M).
Technical Info/Product Notes:LEHD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.

Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
UniProt ID:P55211

Product Literature References

Caspase activation by a zinc-oxide eugenol-based root-canal sealer in cementoblasts: F.M. Huang, et al.; J. Dent. Sci. (2015), Application(s): Fluorometric Assay using murine cementoblasts ,

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