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Nitroso-thiol (RSNOs) detection kit

ALX-850-037-KI01 96 wells 747.00 USD
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The assay of nitroso-thiols in many biological fluids, appears to provide a very useful index of NOS activity. The concentration of RSNOs has been shown to increase with localized induction of NOS and to fall rapidly upon administration of NOS inhibitors.
The kit provides a complete solution for the assay of high or low MW RSNOs. The assay is divided essentially into two steps. In the first step, the sample is prepared according to which subgroup of RSNO is desired. The S-N bond is then cleaved, resulting in the formation of an equivalent of nitrite, which is assayed using the Griess reaction. The intensity of the color formed is linearly related to the concentration of nitrite, and hence, to the original RSNO concentration.

Product Specification

Applications:Colorimetric detection
Quantity:~80 tests
Handling:Store kit at +4°C. Store standard (vial 5) at -20°C. Protect from light.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C

Product Literature References

Dose-dependent onset and cessation of action of inhaled budesonide on exhaled nitric oxide and symptoms in mild asthma: S.A. Kharitonov, et al.; Thorax 57, 889 (2002), Abstract;
Increased nitrosothiols in exhaled breath condensate in inflammatory airway diseases: M. Corradi, et al.; Am. J. Respir. Crit. Care Med. 163, 854 (2001), Abstract; Full Text

General Literature References

Mechanisms of biological S-nitrosation and its measurement: T. Akaike; Free Radic. Res. 33, 461 (2000), Abstract;
Detection of S-nitrosothiols by fluorometric and colorimetric methods: D.A. Wink, et al.; Methods Enzymol. 301, 201 (1999), Abstract;
Fluorometric detection of S-nitrosothiols: P. Kostka and J.K. Park; Methods Enzymol. 301, 227 (1999), Abstract;

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