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Osteoprotegerin (human) monoclonal antibody (Bony-1) (biotin conjugate)

 
ALX-804-813B-C100 100 µg Biotin 310.00 USD
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Product Specification

Alternative Name:OPG, TNFRSF 11B, Osteoclastogenesis inhibitory factor
 
Clone:Bony-1
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant human OPG (osteoprotegerin) cysteine-rich region (aa 22-202) (Prod. No. ALX-522-007).
 
UniProt ID:O00300
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Applications:ELISA, Flow Cytometry, IP
 
Recommended Dilutions/Conditions:Flow Cytometry (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
 
Handling:Do not freeze. Protect from light.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
804-813 02
Figure: FACS analysis of cells with MAb to OPG (human) (Bony-1) (Prod. No. ALX-804-813).
Method: HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to OPG (human) (Bony-1) (Prod. No. ALX-804-813). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
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General Literature References

Osteoprotegerin is a soluble decoy receptor for tumor necrosis factor-related apoptosis-inducing ligand/Apo2 ligand and can function as a paracrine survival factor for human myeloma cells: C.M. Shipman and P.I. Croucher; Cancer Res. 63, 912 (2003), Abstract; Full Text
Osteoprotegerin: U. Feige; Ann. Rheum. Dis. 60, Suppl 3, iii81 (2001), Abstract; Full Text

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