Microcystins (Adda specific) (AD4G2), mAb



ALX-804-585-C100	100 µg	209.00 USD

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody AD4G2, IC₅₀ values for microcystin-LR of 0.06 µg/l have been obtained. The provisional guideline value proposed by the WHO is 1µg/l for drinking water. The detection limit for microcystin-LR is 0.07µg/l. All microcystin variants show similar IC₅₀ values and detection limits. No microcystins are known, that are not recognized by this antibody. Microcystin-LR spiked water samples in the concentration range between 0.1 and 1µg/l were measured and a mean recovery of 113 ± 23% was found.

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Product Specification

Formulation:	Lyophilized.

Clone:	AD4G2

Isotype:	Mouse IgG1

Immunogen:	N-Acetyl-Adda-KLH and N-Acetyl-Adda-BSA.

Reconstitution:	Reconstitute with 100µl PBS containing sodium azide.

Specificity:	Recognizes all microcystins.

Application:	ELISA (1:10'000) Microcystins can be measured at a detection limit of ~0.03µg/l.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Positive Control:	Products No. ALX-350-012, ALX-350-043, ALX-350-080 and ALX-350-081.

Miscellaneous/General:	Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 amino acid 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-amino acids.

Product Literature References

Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci. 17, 1445 (2001), Abstract;

Generic microcystin immunoassay based on monoclonal antibodies against Adda: A. Zeck, et al.; Analyst. 126, 2002 (2001), Abstract;

Highly sensitive immunoassay based on a monoclonal antibody specific for [4-arginine]microcystins: A. Zeck et al.; Anal.Chim. Acta 441, 1 (2001),

Multidimensional biochemical detection of microcystins in liquid chromatography: A. Zeck, et al.; Anal. Chem. 73, 5509 (2001), Abstract;