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Osteocalcin monoclonal antibody (4-9) (OCG4)

 
ALX-804-537-C100 100 µg 668.00 USD
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Product Specification

Clone:OCG4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Bovine osteocalcin.
 
UniProt ID:P02820
 
Species reactivity:Human
Bovine, Chicken, Dog, Goat, Porcine, Rabbit, Sheep
 
Specificity:Recognizes aa 4-9 of osteocalcin.
 
Crossreactivity:Does not cross-react with mouse or rat osteocalcin.
 
Applications:IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Affinity purified.
 
Formulation:Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Does not contain any preservatives.
 
Reconstitution:Reconstitute with 50µl distilled water. Dilute with 10mM PBS, pH 7.4, 1% BSA and 0.1% sodium azide.
 
Use/Stability:Reconstituted solution is stable for 6 months when stored at +4°C (after adding 0.1% sodium azide as a preservative) and for 1 year when stored at -20°C. 
 
Handling:Dilute only prior to immediate use. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-534 804-535 804-536 804-537
Figure: Recognition sites of MAbs to osteocalcin assayed by peptide fragments.
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804-534 804-535 804-536 804-537

Product Literature References

Undercarboxylated osteocalcin measured with a specific immunoassay predicts hip fracture in elderly women: the EPIDOS Study: P. Vergnaud, et al.; J. Clin. Endocrinol. Metab. 82, 719 (1997), Abstract; Full Text
A one step sandwich enzyme immunoassay for gamma-carboxylated osteocalcin using monoclonal antibodies: N. Koyama, et al.; J. Immunol. Methods 139, 17 (1991), Abstract;
Vitamin K-dependent formation of bone Gla protein (osteocalcin) and its function  : P.A. Price; Vitam. Horm. 42, 65 (1985), Abstract;
Characterization of a gamma-carboxyglutamic acid-containing protein from bone : P.A. Price, et al.; PNAS 73, 1447 (1976), Abstract;

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