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Smac/DIABLO (mouse) monoclonal antibody (9H10)

 
ALX-804-366-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:Second mitochondria-derived activator of caspase
 
Clone:9H10
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse Smac/DIABLO (aa 55-237) .
 
UniProt ID:Q9JIQ3
 
Species reactivity:Mouse
 
Applications:ELISA, Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB
 
Application Notes:For the detection of human Smac/DIABLO use MAb to Smac/DIABLO (human) (10G7) (Prod. No. ALX-804-333).
 
Formulation:Liquid. Protein G purified antibody in PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

804-366-2a
Figure: Mouse Smac/DIABLO is associated with the mitochondria when overexpressed in human HeLa cells, nuclei are counterstained with propidium iodide (PI).
Method: MAb to Smac/DIABLO (clone 9H10) was used at a dilution of 1:100 (in C, phase contrast in A, staining with PI in B, overlay with PI shown in D), but the optimal concentration needs to be determined individually. Cells were fixed with 4% PFA and permeabilized with 0.1-0.3% saponin, followed by incubation with an anti-rat-ALEXA490 conjugated antibody (Molecular Probes). Staining with a negative control (rat IgG) is shown in E to H.

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Product Literature References

Treatment of mice with 2,3,7,8-tetrachlorodibenzo-p-dioxin leads to aryl hydrocarbon receptor-dependent nuclear translocation of NF-kappaB and expression of Fas ligand in thymic stromal cells and consequent apoptosis in T cells: I.A. Camacho, et al.; J. Immunol. 175, 90 (2005), Abstract; Full Text
Tissue distribution of Diablo/Smac revealed by monoclonal antibodies: A. Tikoo, et al.; Cell Death Differ. 9, 710 (2002), Abstract;
Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension: T. Kataoka, et al.; J. Biol. Chem. 276, 19548 (2001), Abstract; Full Text

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