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Toll-like receptor 6 (human) monoclonal antibody (86B1153.2)

 
ALX-804-363-C100 100 µg 345.00 USD
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Product Specification

Alternative Name:TLR6, CD286
 
Clone:86B1153.2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 408-424 in the cytoplasmic portion of human TLR6 (Toll-like receptor 6).
 
UniProt ID:Q9Y2C9
 
Species reactivity:Human
 
Applications:Flow Cytometry, IHC
 
Recommended Dilutions/Conditions:Flow Cytometry (intracellular, 0.5-5µg per 106 cells)
Immunohistochemistry (paraffin sections, 5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Flow Cytometry: Suitable for cell surface and intracellular FC.
 
Positive Control:Ramos cells can be used as positive control.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
 
Use/Stability:Stable for at least 6 months when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-363
Figure: Intracellular flow cytometry of TLR6 (shaded peak) and mouse IgG1 isotype control (open peak) in 106 Ramos cells using 3µg of MAb to TLR6 (Prod. No. ALX-804-363).
804-363 4
Figure: Immunohistochemical analysis of TLR6 in paraffin-embedded formalin-fixed human liver tissue using an isotype control (top) or MAb to TLR6 (Prod. No. ALX-804-363) at 5μg/ml.
ALX-804-363 IHC
Immunohistochemistry analysis: Formalin-fixed, paraffin-embedded tonsil probed with Toll-like receptor 6 (human), mAb (86B1153.2) at 5µg/ml.
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804-363 804-363 4 ALX-804-363 IHC

Product Literature References

Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007), Abstract;
Differential interactions of fimbriae and lipopolysaccharide from Porphyromonas gingivalis with the Toll-like receptor 2-centred pattern recognition apparatus: G. Hajishengallis, et al.; Cell Microbiol. 8, 1557 (2006), Abstract;
Toll-like receptor 2-mediated human B cell differentiation: L.M. Ganley-Leal, et al.; Clin. Immunol. 120, 272 (2006), Abstract;
Evidence of Toll-like receptor molecules on human platelets: F. Cognasse, et al.; Immunol. Cell Biol. 83, 196 (2005), Abstract;

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Toll-like receptor 6 (human) monoclonal antibody (86B1153.2) (FITC conjugate) 

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