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TRAIL (human) monoclonal antibody (HS501)

 
ALX-804-300-C100 100 µg 329.00 USD
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Product Specification

Alternative Name:Apo-2L, TNFSF 10, CD253
 
Clone:HS501
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human soluble TRAIL.
 
UniProt ID:P50591
 
GenBank ID:U57059
 
Gene/Protein Identifier:8743 (Entrez GeneID)
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Applications:WB
 
Application Notes:Excellent for Western blot.
 
Formulation:Liquid. In PBS containing PEG-sucrose and 0.02% sodium azide.
 
Handling:Thaw at +37°C. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-80°C
 
804-300-n2

Figure 1: Equal numbers of Jurkat cells treated and untreated with IFNa compared with equal numbers of CV1-EBNA cells transfected with human TRAIL and a control expression plasmid (not encoding hTRAIL) were analyzed by using HS501 (Prod. No. ALX-804-300.Method: Jurkat cells were treated with IFNa [1000 IU/ml] for 8 hours (lane 1) or were left untreated (lane 2), and CV1-EBNA cells were transfected with pcDNA3 expression vector encoding human TRAIL (lane 3), or with human TRAIL-R1 (lane 4). Lysates of equal numbers of cells were analyzed by Western blot with anti-TRAIL HS501 (Prod. No. ALX-804-300). The primary antibody was used at 1µg/ml, followed by horseradish peroxidase-conjugated anti-mouse IgG1 antibody and developed by ECL.

804-300-n1

Figure 2: Equal numbers of CV1-EBNA cells transfected with control expression plasmids (negative for human TRAIL) were analyzed and compared to a positive control cell-lysate using HS501.Method: CV1-EBNA cells, either transfected with pcDNA3 expression vector encoding TRAIL-R1 to TRAIL-R4 (lanes 1-4), or with human TRAIL (lane 5) were analyzed with HS501 MAb against TRAIL (Prod. No. ALX-804-300). The primary antibody was used at 1µg/ml, followed by a horseradish peroxidase conjugated anti-mouse IgG1 antibody and developed by ECL.

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Product Literature References

TNF-Related Apoptosis-Inducing Ligand Mediates Tumoricidal Activity of Human Monocytes Stimulated by Newcastle Disease Virus: B. Washburn, et al.; J. Immunol. 170, 1814 (2003), Abstract;

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