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Cathepsin L/procathepsin L monoclonal antibody (CPLH 3G10)

ALX-804-293-C200 200 µg 590.00 USD
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Product Specification

Clone:CPLH 3G10
Immunogen:Purified human procathepsin L.
UniProt ID:P07711 (cathepsin L1), O60911 (cathepsin L2)
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes the epitope H304CGLATAASY313 of mouse procathepsin L. Recognizes mature mouse single or two chain cathepsin L (light chain) and mouse procathepsin L.
Crossreactivity:Cross-reacts with mature human single or two chain cathepsin L (light chain), human procathepsin L and rat cathepsin L (light chain). Does not cross-react with mature cathepsins B, D, H, K, S and procathepsins B, D, F, S, W.
Applications:ELISA, WB
Recommended Dilutions/Conditions:ELISA (capture antibody)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. Dilutions given in the figures below may be used as guidelines.
Formulation:Liquid. Protein G purified antibody in PBS containing 0.01% thimerosal.
Handling:Do not freeze.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Scientific Background:Cathepsin L [EC] is a lysosomal endopeptidase belonging to the papain family of the closely related cysteine proteases superfamily. The enzyme is ubiquitously distributed in the lysosomes of all cells and seems to be involved in a variety of disorders in which the pathological destruction of the extracellular matrix is one of the main characteristics.
LBS-AB-CL-2005 1
Figure 1: Epitope map of mature cathepsin L.

LBS-AB-CL-2005 6
Figure 6: The antibody can be used as catcher antibody (5µg per ml) in an ELISA for the quantitative determination of mouse cathepsin L (standard curve between 4ng and 500ng cathepsin L per ml).
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LBS-AB-CL-2005 1 LBS-AB-CL-2005 6

Product Literature References

Immunohistochemical and clinical evaluation of cathepsin expression in soft tissue sarcomas: P. Wurl, et al.; Virchows Arch. 430, 221 (1997), Abstract;
Monoclonal antibodies against cathepsin L and procathepsin L of different species: E. Weber, et al.; Hybridoma 16, 159 (1997), Abstract;
Hybridoma cells producing antibodies to cathepsin L have greatly reduced potential for tumour growth: E. Weber, et al.; J. Cancer. Res. Clin. Oncol. 120, 564 (1994), Abstract;

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