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HSP70 monoclonal antibody (3A3)

 
ALX-804-047-R050 50 µl 399.00 USD
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Product Specification

Alternative Name:Heat shock protein 70
 
Clone:3A3
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human HSP70 (heat shock protein 70).
 
UniProt ID:P0DMV8 (HSPA1A), P0DMV9 (HSPA1B)
 
GenBank ID:M11717
 
Gene/Protein Identifier:NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM)
 
Species reactivity:Human, Mouse, Rat
Amphibian, Arthropod, Avian, Drosophila, Fish, Porcine, Plant, Primate, Yeast, Mollusc
 
Specificity:Recognizes several members of the HSP70 gene family including HSP70, HSC70, p75, and following heat shock, HSP72.
 
Crossreactivity:HSP70, HSC70, HSP75, HSP72 (following heat shock).
 
Applications:ICC, IF, IHC (PS), IP, WB
Gel Shift
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (paraffin sections) (1:200)
Immunoprecipitation (2µl)
Western Blot (1:1,000-1:5,000)
Gel Shift (Assay dependent)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Immunocytochemistry: staining of HSP70 in heat shocked HeLa cells results in cytoplasmic staining
Detects bands from ~70kDa to ~78kDa by Western blot representing different members of the HSP70 family.
 
Formulation:Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria.
 
Technical Info/Product Notes:2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts.
 

Product Literature References

A 16-kDa protein functions as a new regulatory protein for Hsc70 molecular chaperone and is identified as a member of the Nm23/nucleoside diphosphate kinase family: S.M. Leung & L.E. Hightower; J. Biol. Chem. 272, 2607 (1997), Abstract; Full Text

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Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
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