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Minor vault p193 protein (human) monoclonal antibody (p193-10)

 
ALX-801-025-C250 250 µg 470.00 USD
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Product Specification

Alternative Name:PARP-4, VPARP
 
Clone:p193-10
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Minor vault p193 protein (VPARP; PARP-4).
 
UniProt ID:Q9UKK3
 
Species reactivity:Human
 
Specificity:Recognizes epitope aa 506-510 (VALGK) of human minor vault p193 protein.
 
Applications:IHC (FS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry: Use 1:10-1:20 dilution for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. Apply the following pretreatment: 10 min in 20mM glycine in PBS (pH 7.5) and 10 min in 6N guanidine hydrochloride in 50mM TRIS-HCl (pH 7.5); Schroeijers et al., (2000).
Western Blot: Use 1:10-1:20 dilution (ECL)
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines: A.B. Schroeijers, et al.; Cancer Res. 60, 1104 (2000), Abstract; Full Text

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