MegaAPRIL™ (soluble) (human), (recombinant)



ALX-522-035-3010	3x10 µg	587.00 USD

Product Specification

Alternative Name:	APRIL:ACRP30headless, TNFSF 13:ACRP30headless, A-proliferation-inducing ligand:ACRP30headless, CD256:ACRP30headless

Concentration:	0.1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~34kDa (SDS-PAGE).

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Biological Activity:	Stimulates proliferation of serum-starved NIH 3T3 cells. Stimulates B cell proliferation.

Source/Host:	Produced in HEK 293 cells. The extracellular domain of human APRIL (aa 88-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG^®-tag.

Reconstitution:	Reconstitute with 100µl sterile water.

Specificity:	Binds to human and mouse BCMA and TACI. Binds to proteoglycans.

Long Term Storage:	-20°C

Use/Stability:	PBS containing at least 0.1% BSA should be used for further dilutions. Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID O75888: APRIL (human) AfCS Signaling Gateway ID A000305: APRIL (mouse) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure: MegaAPRIL™, Soluble (human) (rec.) induces Swiss NIH-3T3 cells to proliferate in low serum conditions.Method: Proliferation assay: 5000 Swiss NIH3T3 cells were plated per well of a 96-well plate and rested overnight. Cells were then washed once in PBS buffer and starved in medium with 0% FCS for 24 hours. After the starvation period, medium containing 0.2% FCS (left column) or containing 0.2% FCS and 20ng/ml of MegaAPRIL™ (right column) was added to the cells. Cells were then cultured for 48 hours and cell viability was determined using a PMS/MTS-based cell proliferation assay kit.

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Product Literature References

BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway: T. Endo, et al.; Blood 109, 703 (2007), Abstract; Full Text

TACI regulates IgA production by APRIL in collaboration with HSPG: D. Sakurai, et al.; Blood 109 , 2961 (2007), Abstract;

APRIL and BAFF promote increased viability of replicating human B2 cells via mechanism involving cyclooxygenase 2: P.K. Mongini, et al.; J. Immunol. 176, 6736 (2006), Abstract; Full Text

BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med. 202, 1363 (2005), Abstract;

Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004), Abstract; Full Text

Potential role of APRIL as autocrine growth factor for megakaryocytopoiesis: D. Bonci, et al.; Blood 104, 3169 (2004), Abstract; Full Text

APRIL modulates B and T cell immunity: J.V. Stein, et al.; J. Clin. Invest. 109, 1587 (2002), Abstract; Full Text

DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL: M.B. Litinskiy, et al.; Nat. Immunol. 3, 822 (2002), Abstract;

General Literature References

B-cell maturation protein, which binds the tumor necrosis factor family members BAFF and APRIL, is dispensable for humoral immune responses: S. Xu & K.P. Lam; Mol. Cell. Biol. 21, 4067 (2001), Abstract;

A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000), Abstract;

APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity: G. Yu, et al.; Nat. Immunol. 1, 252 (2000), Abstract;

Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI: S.A. Marsters, et al.; Curr. Biol. 10, 785 (2000), Abstract;

Tumor necrosis factor (TNF) receptor superfamily member TACI is a high affinity receptor for TNF family members APRIL and BLyS: Y. Wu, et al.; J. Biol. Chem. 275, 35478 (2000), Abstract;

APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth: M. Hahne, et al.; J. Exp. Med. 188, 1185 (1998), Abstract;