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Ac-LEHD-pNA

Caspase-9 substrate
 
ALX-260-081-M001 1 mg 61.00 USD
 
ALX-260-081-M005 5 mg 152.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-P443

Chromogenic substrate for caspase-9 and cysteine proteases. Similar to Ac-LEHD-AMC (Prod. No. ALX-260-080) and Ac-LEHD-AFC (Prod. No. ALX-260-116) but cleavage is monitored colorimetrically by absorbance at 405nm.

Product Specification

Alternative Name:Caspase-9 substrate (chromogenic)
 
Sequence:Ac-Leu-Glu-His-Asp-pNA (pNA = p-Nitroaniline)
 
Formula:C29H38N8O11
 
MW:674.7
 
Formulation:Lyophilized.
 
Peptide Content:75-95%
 
Purity:≥99% (HPLC)
 
Solubility:Soluble in water or  DMSO. Dilute with buffer, pH 7.5.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:λmax of pNA is 400nm.
 
Protocol:HEPES-Buffer: 100 mM HEPES, pH 7.5; 20% (v/v)
 glycerol; 5 mM DTT, 0.5 mM EDTA.

Substrate: Prepare 1 mM stock solution in DMSO.
- Induce apoptosis and prepare cell lysate or use recombinant caspase.
- Prepare reaction solution:
30 µl of substrate stock solution + 240 µl HEPES-Buffer + 30 µl of cell lysate.
- Incubate for 1 hour at 37 °C.
- Measure with microplate reader at 400nm.
- Suggested controls: Reaction mixture…
…without substrate.
 …with non-apoptotic cell lysate.
 …with apoptotic cell lysate and caspase inhibitor.
 

Product Literature References

Early effects of Aβ1-42 oligomers injection in mice: Involvement of PI3K/Akt/GSK3 and MAPK/ERK1/2 pathways: F. Morroni, et al.; Behav. Brain Res. 314, 106 (2016), Application(s): Determination of caspase-9 activation in tissue lysates, Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry et al.; J. Biol. Chem. 272, 17907 (1997), Abstract;

Related Products

Ac-LEHD-AMC 

Caspase-9 substrate
292633-16-0, ≥98% | Print as PDF
 
ALX-260-080-M001 1 mg 51.00 USD
 
ALX-260-080-M005 5 mg 144.00 USD
Do you need bulk/larger quantities?
 

Ac-LEHD-AFC 

Caspase-9 substrate
210345-03-2, ≥96% (HPLC) | Print as PDF
 
ALX-260-116-M005 5 mg 194.00 USD
 
ALX-260-116-M010 10 mg 338.00 USD
Do you need bulk/larger quantities?
 

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