Replaces Prod. #: BML-P444
Fluorogenic substrate for caspase-9 and related cysteine proteases. The LEHD sequence is derived from combinatorial experiments. Ex.:340-360nm, Em.:440-460nm.
Product Details
| Alternative Name: | Caspase-9 substrate (fluorogenic) |
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| Sequence: | Ac-Leu-Glu-His-Asp-AMC (AMC=7-Amino-4-methylcoumarin) |
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| Formula: | C33H41N7O11 |
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| MW: | 711.7 |
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| CAS: | 292633-16-0 |
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| Peptide Content: | 75-95% |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in water, DMSO or phosphate buffer, pH 7.5. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Handling: | Keep cool and dry. |
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| Technical Info/Product Notes: | AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Autophagy displays divergent roles during intermittent amino acid starvation and toxic stress-induced senescence in cultured skeletal muscle cells: D. Bloemberg & J. Quadilatero; J. Cell. Physiol.
236, 3099 (2021),
Application(s): Caspase-9 activity assay on cell lysates,
Abstract;
Study of commonly used organophosphate pesticides that induced oxidative stress and apoptosis in peripheral blood lymphocytes of rats: A. Ojha, et al.; Hum. Exp. Toxicol.
36, 1158 (2017),
Abstract;
The effect of ARC ablation on skeletal muscle morphology, function, and apoptotic signaling during aging: K. Vorobej, et al.; Exp. Gerontol.
101, 69 (2017),
Abstract;
Data on skeletal muscle apoptosis, autophagy, and morphology in mice treated with doxorubicin: T.L. Campbell, et al.; Data Brief
7, 786 (2016),
Application(s): Assessed caspase-3, -8, and -9 activity in mixed gastrocnemius homogenates,
Abstract;
Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro: F. Brisdelli, et al.; Life Sci.
158, 89 (2016),
Application(s): Caspase activity,
Abstract;
Ruthenium Complexes Induce HepG2 Human Hepatocellular Carcinoma Cell Apoptosis and Inhibit Cell Migration and Invasion through Regulation of the Nrf2 Pathway: Y. Lu, et al.; Int. J. Mol. Sci.
17, E775 (2016),
Application(s): Caspase activation by Ru complexes,
Abstract;
Full Text
Treatment of Mycobacterium tuberculosis-Infected Macrophages with Poly(Lactic-Co-Glycolic Acid) Microparticles Drives NFκB and Autophagy Dependent Bacillary Killing: C. Lawlor, et al.; PLoS One
11, e0149167 (2016),
Application(s): Caspase activity assays,
Abstract;
Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
300, R531 (2011),
Abstract;
Full Text
Interaction of polyunsaturated fatty acids and sodium butyrate during apoptosis in HT-29 human colon adenocarcinoma cells: J. Hofmanova; Eur. J. Nutrition
44, 40 (2005),
Abstract;
Bid truncation, bid/bax targeting to the mitochondria, and caspase activation associated with neutrophil apoptosis are inhibited by granulocyte colony-stimulating factor: N.A. Maianski, et al.; J. Immunol.
172, 7024 (2004),
Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
