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Caspase-9 substrate
ALX-260-116-M005 5 mg 256.00 USD
ALX-260-116-M010 10 mg 445.00 USD
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Replaces Prod. #: BML-P445

Fluorogenic substrate for caspase-9.

Product Details

Alternative Name:Caspase-9 substrate (fluorogenic)
Sequence:Ac-Leu-Glu-His-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin)
Purity:≥96% (HPLC)
Appearance:White to off-white powder.
Solubility:Soluble in DMSO.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Handling:Protect from light.
Technical Info/Product Notes:AFC has an excitation maximum of 400nm and an emission maximum of 505nm.
Regulatory Status:RUO - Research Use Only

Product Literature References

CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout: S. Devi, et al.; Cell Rep. 42, 112265 (2023), Abstract;
Caspase-9 Activation of Procaspase-3 but Not Procaspase-6 Is Based on the Local Context of Cleavage Site Motifs and on Sequence: I.V. Soni & J.A. Hardy; Biochemistry 60, 2824 (2021), Abstract;
Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling: L.E. Araya, et al.; ACS Chem. Biol. 16, 2280 (2021), Abstract;
Exogenous Introduction of Initiator and Executioner Caspases Results in Different Apoptotic Outcomes: F. Anson, et al.; JACS Au 1, 1240 (2021), Abstract;
C33-A cells transfected with E6*I or E6*II the short forms of HPV-16 E6, displayed opposite effects on cisplatin-induced apoptosis: C.E. Vaisman, et al.; Virus Res. 247, 94 (2018), Abstract;
CRISPR knock out of programmed cell death protein 1 enhances anti-tumor activity of cytotoxic T lymphocytes: Z. Zhao, et al. ; Oncotarget 9, 5208 (2018), Abstract; Full Text
Nimbolide reduces CD44 positive cell population and induces mitochondrial apoptosis in pancreatic cancer cells: S. Kumar, et al.; Cancer Lett. 413, 82 (2017), Abstract;
Sub-lethal oxidative stress induces lysosome biogenesis via a lysosomal membrane permeabilization-cathepsin-caspase 3-transcription factor EB-dependent pathway: S.M. Leow, et al.; Oncotarget 8, 16170 (2017), Abstract; Full Text
Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells: Y. Pang, et al.; Exp. Cell Res. 16, 30161 (2016), Application(s): Fluorometric assay for caspase-9 activity, Abstract;
Mechanism of neem limonoids-induced cell death in cancer: Role of oxidative phosphorylation: N. Yadav, et al.; Free Radic. Biol. Med. 90, 261 (2016), Application(s): Quantification of apoptosis and caspase activity measurement, Abstract;
Oxidative phosphorylation-dependent regulation of cancer cell apoptosis in response to anticancer agents: N. Yadav, et al.; Cell Death Dis. 5, e1969 (2015), Application(s): Cell culture, Fluorescence, Abstract;
Redox regulation of metabolic and signaling pathways by thioredoxin and GLUTAREDOXIn in nos-3 overexpressing hepatoblastoma cells: R. González, et al.; Redox Biol. 6, 122 (2015), Application(s): Cell Culture, Fluorescence, Abstract;

General Literature References

A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract;

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