Replaces Prod. #: BML-P445
Fluorogenic substrate for caspase-9.
Product Details
| Alternative Name: | Caspase-9 substrate (fluorogenic) |
| |
| Sequence: | Ac-Leu-Glu-His-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin) |
| |
| Formula: | C33H38F3N7O11 |
| |
| MW: | 765.7 |
| |
| CAS: | 210345-03-2 |
| |
| Formulation: | Lyophilized. |
| |
| Purity: | ≥96% (HPLC) |
| |
| Appearance: | White to off-white powder. |
| |
| Solubility: | Soluble in DMSO. |
| |
| Shipping: | Ambient Temperature |
| |
| Long Term Storage: | -20°C |
| |
| Handling: | Protect from light. |
| |
| Technical Info/Product Notes: | AFC has an excitation maximum of 400nm and an emission maximum of 505nm. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Caspase-9 Activation of Procaspase-3 but Not Procaspase-6 Is Based on the Local Context of Cleavage Site Motifs and on Sequence: I.V. Soni & J.A. Hardy; Biochemistry
60, 2824 (2021),
Abstract;
Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling: L.E. Araya, et al.; ACS Chem. Biol.
16, 2280 (2021),
Abstract;
Exogenous Introduction of Initiator and Executioner Caspases Results in Different Apoptotic Outcomes: F. Anson, et al.; JACS Au
1, 1240 (2021),
Abstract;
C33-A cells transfected with E6*I or E6*II the short forms of HPV-16 E6, displayed opposite effects on cisplatin-induced apoptosis: C.E. Vaisman, et al.; Virus Res.
247, 94 (2018),
Abstract;
CRISPR knock out of programmed cell death protein 1 enhances anti-tumor activity of cytotoxic T lymphocytes: Z. Zhao, et al. ; Oncotarget
9, 5208 (2018),
Abstract;
Full Text
Nimbolide reduces CD44 positive cell population and induces mitochondrial apoptosis in pancreatic cancer cells: S. Kumar, et al.; Cancer Lett.
413, 82 (2017),
Abstract;
Sub-lethal oxidative stress induces lysosome biogenesis via a lysosomal membrane permeabilization-cathepsin-caspase 3-transcription factor EB-dependent pathway: S.M. Leow, et al.; Oncotarget
8, 16170 (2017),
Abstract;
Full Text
Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells: Y. Pang, et al.; Exp. Cell Res.
16, 30161 (2016),
Application(s): Fluorometric assay for caspase-9 activity,
Abstract;
Mechanism of neem limonoids-induced cell death in cancer: Role of oxidative phosphorylation: N. Yadav, et al.; Free Radic. Biol. Med.
90, 261 (2016),
Application(s): Quantification of apoptosis and caspase activity measurement,
Abstract;
Oxidative phosphorylation-dependent regulation of cancer cell apoptosis in response to anticancer agents: N. Yadav, et al.; Cell Death Dis.
5, e1969 (2015),
Application(s): Cell culture, Fluorescence,
Abstract;
Redox regulation of metabolic and signaling pathways by thioredoxin and GLUTAREDOXIn in nos-3 overexpressing hepatoblastoma cells: R. González, et al.; Redox Biol.
6, 122 (2015),
Application(s): Cell Culture, Fluorescence,
Abstract;
General Literature References
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
