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 United States

Goat anti-mouse IgG1 (human & mouse Ig adsorbed), (HRP conjugate)

 
ALX-211-203-R050 50 µl 171.00 USD
 
A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtain good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immunoglobulins will enhance results.

Product Specification

Concentration:1mg/ml.
 
Purity Detail:Purified by affinity chromatography on mouse IgG1 covalently linked to agarose. Cross-adsorbed with mouse IgM, IgG2a, IgG2b, IgG3, IgA and human serum.
 
Formulation:Liquid. In PBS, pH 7.4, containig 50% glycerol. Contains no preservatives.
 
Immunogen:Mouse IgG1.
 
Source/Host:From goat.
 
Specificity:Recognizes the heavy chain of mouse IgG1. Minimally cross-reacts with human immunoglobulins.
 
Application:ELISA (1:4'000-1:8'000)
Western Blot (1:5'000)
Optimal conditions must be determined individually for each application.
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Handling:After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Protect from light.
 

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