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Adiponectin receptor 1 (human) polyclonal antibody (AL238)

 
ALX-210-913-R100 100 µl 371.00 USD
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Product Specification

Alternative Name:AdipoR1, CGI-45
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 2-36 (S2SHKGSVVAQGN
GAPASNREADTVELAELGPLLEE36) of human adiponectin receptor 1 (AdipoR1).
 
UniProt ID:Q96A54
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1:100)
Western blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. Neat serum.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
210-913
Figure 1: Detection of Adiponectin Receptor 1 (human) in HEK 293T transfected cells by the PAb to Adiponectin Receptor 1 (human) (AL238) (Prod. No. ALX-210-913).Method: 10µg per lane of total cell extracts from mock transfected HEK 293T cells (lanes 1 and 3) or HEK 293T cells transfected with an expression vector encoding the Flag-tagged human AdipoR1 (lanes 2 and 4) were run on a 12% SDS-PAGE gel and analyzed by Western blot. Lanes 1 and 2 were probed with an anti-FLAG antibody at a 1:2’000 dilution. Lanes 3 and 4 were probed with AL238 at a 1:1’000 dilution. After 1 hour of incubation at RT, the membrane was extensively washed and then incubated with goat anti-mouse horseradish peroxydase (Prod. No. APO-26G-050H) or a goat anti-rabbit horseradish peroxydase (Jackson, Prod. No. 111-035-144), respectively, for ECL detection. Note that the upper bands might represent oligomerized forms of the receptor.
210-913 figure2
Figure 2: Paraffin embedded human adipose tissue stained with PAb to Adiponectin Receptor 1 (human) (AL238) (Prod. No. ALX-210-913).
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210-913 210-913 figure2

General Literature References

Cloning of adiponectin receptors that mediate antidiabetic metabolic effects: T. Yamauchi, et al.; Nature 423, 762 (2003), Abstract;

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