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Nampt (Visfatin/PBEF) (human) polyclonal antibody

 
ALX-210-425-C100 100 µg 320.00 USD
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Product Specification

Alternative Name:PBEF1, Pre-B-cell colony enhancing factor 1, Nicotinamide phosphoribosyltransferase
 
Host:Rabbit
 
Immunogen:Recombinant human Nampt (visfatin/PBEF).
 
UniProt ID:P43490
 
Species reactivity:Human
 
Crossreactivity:Weakly cross-reacts with mouse Nampt.
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (direct and indirect, 1:2,000-1:5,000)
Immunohistochemistry (paraffin sections, 1:500-1:1,000)
Western Blot (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Purified IgG.
 
Formulation:Liquid. In PBS, pH 7.4. Contains no preservatives.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Nampt (Visfatin/PBEF) (human) polyclonal antibody Western blot
Figure: Western blot analysis of human Nampt using Nampt (Visfatin/PBEF) (human), pAb (Prod. No. ALX-210-425) at 1:2’000 dilution.Lane 1: Recombinant human Nampt (His tagged) (Prod. No. ALX-201-319)Lane 2: LPS-treated human peripheral blood leukocyte lysate"
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Nampt (Visfatin/PBEF) (human) polyclonal antibody Western blot

Product Literature References

Visfatin, an adipocytokine with proinflammatory and immunomodulating properties: A.R. Moschen, et al.; J. Immunol. 178, 1748 (2007), Abstract;
Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett. 580, 4105 (2006), Abstract;

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