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Nitric oxide synthase (neuronal) (oxygenase domain) (human), (recombinant) (purified)

 
ALX-201-264-C250 250 µg 435.00 USD
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Product Specification

Alternative Name:nNOS, NOS I
 
MW:~80kDa
 
Source:Produced in E. coli.
 
EC:1.14.13.39
 
UniProt ID:P29475
 
Concentration:10mg/ml
 
Formulation:Liquid. In 40mM bis-tris-propane, pH 7.8, containing 150mM sodium chloride and 0.1% BSA.
 
Purity:≥90% (SDS-PAGE and heme spectral analysis)
 
Specific Activity:≥35nmol/mg/mol protein. Amount assayed 150–500nM. Optimal conditions: 37°C, 40mM EPPS buffer, pH 7.6, 1mM NOHA, 0.5mM DTT, 30mM H2O2, 10units/ml superoxide dismutase, 0.5mg/ml BSA and variable concentrations of tetrahydrobioterin (0–1'000mM) in a final volume of 100ml. Reactions were initiated by 30mM H2O2 and stopped by 1'500 units of catalase.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 1 year after receipt when stored at -80°C. Keep stock vial on ice at all times!
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 

Product Literature References

Nitric-oxide synthase output state. Design and properties of nitric-oxide synthase oxygenase/FMN domain constructs: D.K. Ghosh, et al.; J. Biol. Chem. 281, 14173 (2006), Abstract; Full Text
Structures of nitric oxide synthase isoforms complexed with the inhibitor AR-R17477 suggest a rational basis for specificity and inhibitor design: R. Fedorov, et al.; PNAS 101, 5892 (2004), Abstract; Full Text

General Literature References

High-level expression of functional rat neuronal nitric oxide synthase in Escherichia coli: L.J. Roman, et al.; PNAS 92, 8428 (1995), Abstract; Full Text
Nitric oxide synthases reveal a role for calmodulin in controlling electron transfer: H.M. Abu-Soud and D.J. Stuehr; PNAS 90, 10769 (1993), Abstract; Full Text

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