Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Ubc9 (human), (recombinant)

Conjugating enzyme (E2)
 
ALX-201-046-C050 50 µg 239.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Source:Produced in E. coli.
 
EC:6.3.2.19
 
UniProt ID:P63279
 
Concentration:1.2mg/ml
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 5mM β-mercaptoethanol.
 
Purity:≥95%
 
Application Notes:Ubc9 catalyzes formation of an isopeptide bond between the C-terminus of SUMO-1/sentrin-1 and ε-amino groups in the target protein.

Standard reaction for protein conjugation (10µl) includes the target protein, SUMO-1 activating enzyme (Prod. No. BML-UW9330) (100ng), Ubc9 (900ng) (Prod. No. ALX-201-046), SUMO-1 (1-97) (Prod. No. ALX-201-045) (10µg) in 50mM TRIS, pH 7.6, 5mM magnesium chloride, 2mM ATP, 10mM creatine phosphate, 3.5U/ml of creatine kinase and 0.6U/ml of inorganic pyrophosphatase.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 

Product Literature References

SUMO-1 modification activates the transcriptional response of p53: M.S. Rodriguez, et al.; EMBO J. 18, 6455 (1999), Abstract; Full Text
SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation: J.M. Desterro, et al.; Mol. Cell 2, 233 (1998), Abstract;
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett. 417, 297 (1997), Abstract;

Related Products

SUMO activating enzyme E1 (human), (recombinant) 

Produced in E. coli. Full length heterodimeric human SUMO-activating enzyme. | Print as PDF
 
BML-UW9330-0025 25 µg 261.00 USD
Do you need bulk/larger quantities?
 

SUMO-1 (human) (1-97), (recombinant) 

Produced in E. coli., ≥95% (SDS-PAGE) | Print as PDF
 
ALX-201-045-C500 500 µg 333.00 USD
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

All new literature pieces

Recommend this page

 
Keep in touch

©2017 Enzo Life Sciences, Inc.,