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[pSer326]HSF1 polyclonal antibody

ADI-SPA-902-D 50 µg 215.00 USD
ADI-SPA-902-F 200 µg 466.00 USD
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Product Specification

Alternative Name:HSTF1, Heat shock factor protein 1
Immunogen:Synthetic peptide corresponding to the N-terminus of human HSF1 phosphorylated at Ser 326.
UniProt ID:Q00613
Species reactivity:Human
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Predicted MW of ~60kDa. Detects a band of 85-95kDa by Western blot depending on hyperphosphorylation of the native protein.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.
SPA-902 WB1
Western blot analysis of HSF1 (pSer326): Lane 1: MW marker, Lane 2: HeLa, Lane 3: HeLa, Heat-Shocked (No recovery time), probed with HSF1 (pSer326) pAb.
SPA-902 WB2
Western Blot analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.
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SPA-902 WB1 SPA-902 WB2

Product Literature References

Branched-chain amino acid catabolism is a conserved regulator of physiological ageing: J. Mansfeld, et al.; Nat. Commun. 6, 10043 (2015), Application(s): Immunoblotting, supernatants from nematodes, Abstract; Full Text

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