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HSP70/HSP72 monoclonal antibody (C92F3A-5)

 
ADI-SPA-810-D 50 µg 194.00 USD
 
ADI-SPA-810-F 200 µg 399.00 USD
 
ADI-SPA-810-J 1 mg 1,671.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-SA660

  • Validated for multiple applications
  • Multi-species reactive
  • Widely cited

Product Specification

Alternative Name:Hsp70, HspA1A, Heat shock protein 70, HspA1B, Hsp72
 
Clone:C92F3A-5
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Native human Hsp70 protein.
 
UniProt ID:P0DMV8 (HSPA1A), P0DMV9 (HSPA1B)
 
GenBank ID:M11717
 
Gene/Protein Identifier:NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM)
 
Species reactivity:Human, Mouse, Rat
Beluga, Bovine, C. elegans, Chicken, Dog, Drosophila, Fish, Gerbil, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Teal, Xenopus
 
Applications:ELISA, Flow Cytometry, ICC, IF, IHC, IP, WB
Electron microscopy
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~70kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
 
SPA-810 structure
Substrate binding domain of HSP70 in complex with a substrate peptide10.
SPA-810 IHC
Immunohistochemistry analysis of human colon cancer tissue using HSP70 mAb (C92F3A-5) at a dilution of 1:50.
ADI-SPA-810 WB 01
Western blot analysis of HSP70/HSP72, mAb (C92F3A-5) (Prod. No. ADI-SPA-810): Lane 1: MW marker, Lane 2: HSP70/HSP72 (human), (recombinant) (Prod. No. ADI-NSP-555), Lane 3: HSC70/HSP73 (bovine), (recombinant) (Prod. No. ADI-SPP-751) (Negative Control), Lane 4: PC-12 (heat shocked) (Prod. No. ADI-ADI-LYC-PC101), Lane 5: HeLa (heat shocked) (Prod. No. ADI-LYC-HL101), Lane 6: 3T3 (heat shocked) (Prod. No. ADI-LYC-3T101), Lane 7: CHO-K1 (heat shocked).
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SPA-810 structure SPA-810 IHC ADI-SPA-810 WB 01

Product Literature References

Evaluation of geranylgeranylacetone against cisplatin-induced ototoxicity by auditory brainstem response, heat shock proteins and oxidative levels in guinea pigs: W.C. Lo, et al.; Neurotoxicol Teratol. 61, 29 (2017), Abstract;
Immunoproteomic identification and characterization of Ni2+-regulated proteins implicates Ni2+ in the induction of monocyte cell death: A. Jakob, et al.; Cell Death Dis. 8, e2684 (2017), Abstract; Full Text
Mass spectrometry analyses of normal and polyglutamine expanded ataxin-3 reveal novel interaction partners involved in mitochondrial function: L.V. Kristensen, et al.; Neurochem. Int. 112, 5 (2017), Abstract;
Secretion of cytokines and heat shock protein (HspA1A) by ovarian cancer cells depending on the tumor type and stage of disease: M. Nowak, et al.; Cytokine 89, 136 (2017), Application(s): Western blot, Abstract;
The ubiquitin conjugating enzyme Ube2W regulates solubility of the Huntington's disease protein, huntingtin: B. Wang, et al.; Neurobiol. Dis. 109, 127 (2017), Application(s): Western Blot, Abstract;
Trimethylamine-N-oxide, as a risk factor for atherosclerosis, induces stress in J774A.1 murine macrophages: A. Mohammadi, et al.; Adv. Med. Sci. 63, 57 (2017), Application(s): Western Blot, Abstract;
Addition of Alanyl-Glutamine to Dialysis Fluid Restores Peritoneal Cellular Stress Responses - A First-In-Man Trial: K. Kratochwill, et al.; PLoS One 11, e0165045 (2016), Application(s): Western blot, peritoneal cells, Abstract; Full Text
ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation: J.H. Seo, et al.; Nat. Commun. 7, 12882 (2016), Application(s): In vitro acetylation assay, deacetylation assay, binding assay, and ATPase activity assay, Abstract; Full Text
Compression loading-induced stress responses in intervertebral disc cells encapsulated in 3D collagen constructs: W.H. Chooi, et al.; Sci. Rep. 6, 26449 (2016), Application(s): Immunofluorescence staining, Abstract; Full Text
DHA-induced stress response in human colon cancer cells-focus on oxidative stress and autophagy: K. Pettersen, et al.; Free Radic. Biol. Med. 90, 158 (2016), Application(s): Cell culture, Abstract;
Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins: S. Karvinen, et al.; Exp. Gerontol. 79, 46 (2016), Application(s): Immunoblotting, Abstract;
Functional effects of milk bioactive peptides on skeletal muscle of rats: C.S. Moura, at al.; Food Res. Int. 84, 18 (2016), Application(s): Western blot,
Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping: N. Frara, et al.; BMC Musculoskelet. Dis. 17, 34 (2016), Application(s): Western blot, Abstract; Full Text
IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis: Y. Asano, et al.; Sci. Rep. 12, 19174 (2016), Application(s): Western blot, Abstract; Full Text
Lysosomal membrane stability plays a major role in the cytotoxic activity of the anti-proliferative agent, di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT): E.M. Gutierrez, et al.; Biochim. Biophys. Acta 1863, 1665 (2016), Application(s): Western blotting and immunoprecipitation, Abstract;
Nondamaging Retinal Laser Therapy: Rationale and Applications to the Macula: D. Lavinsky, et al.; Invest. Ophthalmol. Vis. Sci. 57, 2488 (2016), Application(s): Immunohistochemistry, Abstract; Full Text
Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors: A.M. Jaeger, et al.; Nat. Struct. Mol. Biol. 23, 147 (2016), Application(s): Immunoblot analysis, Abstract;
The Molecular Chaperone Hsp70 Promotes the Proteolytic Removal of Oxidatively Damaged Proteins by the Proteasome: S. Reeg, et al.; Free Radic. Biol. Med. 99, 153 (2016), Application(s): Immunoblot Analysis; Immunoprecipitation of Hsp70 from HT22 cell lysates and murine brain lysates, Abstract;
Tissue oxidative stress induced by Patulin and protective effect of Crocin: M. Boussabbeh, et al.; Neurotoxicology 53, 343 (2016), Abstract;
Acute isoproterenol induces anxiety-like behavior in rats and increases plasma content of extracellular vesicles: G. Leo, et al.; Physiol. Behav. 142C, 79 (2015), Application(s): Western Blot, Abstract;
Androgen Deprivation and Cytoprotective Parameters in Rat Lung: S.M. Álvarez, et al.; IJRSB 3, 150 (2015), Application(s): Immunohistochemistry, Full Text
Autophagic degradation of the androgen receptor mediated by increased phosphorylation of p62 suppresses apoptosis in hypoxia: T. Mitani, et al.; Cell. Signal. 27, 1994 (2015), Application(s): Western Blot, Abstract;
Basal and stress-inducible expression of HSPA6 in human keratinocytes is regulated by negative and positive promoter regions: V.P. Ramirez, et al.; Cell Stress Chaperones 20, 95 (2015), Abstract;
Centrosome-intrinsic mechanisms modulate centrosome integrity during fever: A. Vertii, et al.; Mol. Biol. Cell 26, 3451 (2015), Application(s): Cell culture , Abstract; Full Text
Combination of PEI-Mn0.5Zn0.5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma: Q. Tang, et al.; Int. J. Nanomedicine 10, 7129 (2015), Abstract; Full Text
Cross-Omics Comparison of Stress Responses in Mesothelial Cells Exposed to Heat- versus Filter-Sterilized Peritoneal Dialysis Fluids: K. Katochwill, et al.; Biomed Res. Int. 2015, Article ID 628158 (2015), Application(s): Western Blot, Abstract; Full Text
Differential expression patterns among heat-shock protein genes and thermal responses in the whitefly Bemisia tabaci(MEAM 1): F. Diaz, et al.; J. Therm. Biol. 52, 199 (2015), Application(s): Western Blot, Abstract;
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
Dynamic m6A mRNA methylation directs translational control of heat shock response: J. Zhou, et al.; Nature 526, 591 (2015), Application(s): Immunoblotting, Abstract;
Genomic and proteomic investigation of preeclampsia: H. Sahin, et al.; Exp. Ther. Med. (2015), Application(s): Western Blot, Full Text
Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines: F. Clemente-Vicario, et al.; PLoS One 10, e0142007 (2015), Application(s): Immunoblot analyses, Abstract; Full Text
Linking HSP90 target occupancy to HSP70 induction and efficacy in mouse brain: K. Thirstrup, et al.; Pharmacol. Res. 104, 197 (2015), Application(s): Immunoblot analysis using mouse cell and tissue homogenates, Abstract;
Mechanism of Intracellular Communication During Yersinia Pestis Infection: F. Alem; (2015), (master thesis), Application(s): Cytoskeletal Rearrangement, Cell Migration, and Vesicle Trafficking, Abstract; Full Text
Merging colloidal nanoplasmonics and surface plasmon resonance spectroscopy for enhanced profiling of multiple myeloma-derived exosomes: D.N. Giuseppe, et al.; Biosens. Bioelectron. 77, 518 (2015), Application(s): Western Blot, Abstract;
Selective astrocytic endothelin-1 overexpression contributes to dementia associated with ischemic stroke by exaggerating astrocyte-derived amyloid secretion: V. K. Hung, et al.; J. Cereb. Blood Flow Metab. 35, 1687 (2015), Application(s): Western Blot, Abstract;
Sensitizing tumor cells to radiation by targeting the heat shock response: D. Schilling, et al.; Cancer Lett. 360, 294 (2015), Application(s): Western Blotting, Abstract;
Short-Wave Diathermy Pretreatment and Inflammatory Myokine Response After High-Intensity Eccentric Exercise: J. P. Vardiman, et al.; J. Athl. Train. 50, (2015), Application(s): Cell Culture, Abstract; Full Text
Structure-Activity Relationship in a Purine-Scaffold Compound Series with Selectivity for the Endoplasmic Reticulum Hsp90 Paralog Grp94: H. J. Patel, et al.; J. Med. Chem. 58, 3922 (2015), Application(s): Western Blot, Abstract; Full Text
Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia: D. Brea, et al.; Sci. Rep. 5, 12030 (2015), Application(s): Immunohistochemistry, Abstract; Full Text
Thiopental protects human neuroblastoma cells from apoptotic cell death - Potential role of heat shock protein 70: M. Roesslein, et al.; Life Sci. 139, 40 (2015), Application(s): Gel electrophoresis, Western Blotting, Abstract;
TNIP1 reduction of HSPA6 gene expression occurs in promoter regions lacking binding sites for known TNIP1-repressed transcription factors: V.P. Ramirez, et al.; Gene 555, 430 (2015), Application(s): Western blot using human keratinocyte HaCaT cell lysates, Abstract;
Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats: R.R. Ahmed, et al.; Biol. Res. 48, 54 (2015), Application(s): Immunohistochemistry, Abstract; Full Text
Effects of vitamin C and E supplementation on endogenous antioxidant systems and heat shock proteins in response to endurance training: K.T. Cumming, et al.; Physiol. Rep. 2, e12142 (2014), Application(s): Western blot using human muscle tissue lysates, Abstract; Full Text
Determining the Contributions of Caspase-2, Caspase-8 and Effector Caspases to Intracellular VDVADase Activities during Apoptosis Initiation and Execution: M.E. Delgado, et al.; Biochim. Biophys. Acta 1833, 2279 (2013), Application(s): WB using human cell lysates, Abstract;
In vivo theranostics at the peri-infarct region in cerebral ischemia: J. Agulla, et al.; Theranostics 4, 90 (2013), Application(s): IHC, WB of rat brain, Abstract; Full Text
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text
Daily quadratic trend in basal monocyte expressed HSP72 in healthy human subjects: L. Taylor, et al.; Amino Acids 38, 1483 (2010), Abstract;
Methylglyoxal alters the function and stability of critical components of the protein quality control: C. F. Bento, et al.; PLoS One 5, e13007 (2010), Application(s): WB using human cell lysates, Abstract; Full Text
The effect of acute hypoxia on heat shock protein 72 expression and oxidative stress in vivo: L. Taylor, et al.; Eur. J. Appl. Physiol. 109, 849 (2010), Abstract;
Hsp90 charged-linker truncation reverses the functional consequences of weakened hydrophobic contacts in the N domain: S. Tsutsumi, et al. ; Nat. Struct. Mol. Biol. 16, 1141 (2009), Application(s): WB, IP using mouse cell lysates, Abstract;
Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat Shock Proteins and Viral Tumor Antigens: H. Kasamatsu, et al. ; J. Virol. 83, 37 (2008), Application(s): WB, IP, IHC using monkey cell lysates, Abstract;
Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens: P.P. Li, et al.; J. Virol. 83, 37 (2008), Abstract;
Hypertonic saline increases gammadeltaT cell-mediated killing of activated neutrophils: M.I. Hirsh, et al.; Crit. Care Med. 36, 3220 (2008), Abstract;
Induction of Cellular Stress Overcomes the Requirement of Herpes Simplex Virus Type 1 for Immediate-Early Protein ICP0 and Reactivates Expression from Quiescent Viral Genomes: C. Preston, et al. ; J. Virol. 82, 11775 (2008), Application(s): WB using human cell lysates, Abstract;
Neurodegeneration and cellular stress in the retina and optic nerve in rat cerebral ischemia and hypoperfusion models: G. Kalesnykas, et al. ; Neuroscience 155, 937 (2008), Application(s): IHC using rat tissue, Abstract;
Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation: P. Jaakkola, et al. ; Mol. Biol. Cell 19, 2231 (2008), Application(s): ICC using human tissue culture, Abstract;
Exogenous androgen treatment delays the stress response following hamster facial nerve injury: K. Jones, et al. ; J. Neuroendocrinol. 19, 383 (2007), Application(s): IHC using hamster tissue, Abstract;
fMLP induces Hsp27 expression, attenuates NF-κB activation, and confers intestinal epithelial cell protection: E. Chang, et al. ; Am. J. Physiol. Gastrointest. Liver Physiol. 292, G1070 (2007), Application(s): WB using human tissue, Abstract;
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R844 (2007), Application(s): WB using human vastus lateralis muscle tissue, Abstract; Full Text
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: S. Chen & I.R. Brown; Cell Stress Chaperones 12, 51 (2007), Application(s): WB using rat tissue, Abstract;
Persistent sub-lethal chlorine exposure augments temperature induced immunosuppression in Cyprinus carpio advanced fingerlings: A. Pal, et al. ; Fish Shellfish Immunol. 22, 547 (2007), Application(s): WB using fish samples, Abstract;
Abdominal temperature induces region-specific p53-independent apoptosis in the cauda epididymidis of the mouse: M. Jara, et al.; Biol. Reprod. 67, 1189 (2002), Application(s): WB using mouse tissue homogenates, Abstract; Full Text
Chemical preconditioning with 3-nitropropionic acid: lack of induction of neuronal tolerance in gerbil hippocampus subjected to transient forebrain ischemia: P. Garnier, et al.; Brain Res. Bull. 58, 33 (2002), Abstract;
Xenopus small heat shock proteins, Hsp30C and Hsp30D, maintain heat- and chemically denatured luciferase in a folding-competent state: R. Abdulle, et al.; Cell Stress Chaperones 7, 6 (2002), Abstract;
Presence of both constitutive and inducible forms of heat shock protein 70 in the cerebral cortex and hippocampal synapses: I.S. Moon, et al.; Cereb. Cortex 11, 238 (2001), Abstract;
Suppression of heat shock protein-70 by ceramide in heat shock-induced HL-60 cell apoptosis: T. Kondo, et al.; J. Biol. Chem. 275, 8872 (2000), Abstract;
The ubiquitin-related BAG-1 provides a link between the molecular chaperones Hsc70/Hsp70 and the proteasome: J. Luders, et al.; J. Biol. Chem. 275, 4613 (2000), Abstract;
Chaperone-mediated protein folding: A.L. Fink; Physiol. Rev. 79, 425 (1999), Abstract;
Two distinct domains in hsc70 are essential for the interaction with the synaptic vesicle cysteine string protein: B. Stahl, et al.; Eur. J. Cell Biol. 78, 375 (1999), Abstract;
Chaperone suppression of aggregation and altered subcellular proteasome localization imply protein misfolding in SCA1: C.J. Cummings, et al.; Nat. Genet. 19, 148 (1998), Abstract;
Detection of heat shock protein 70 (Hsp70) and anti-Hsp70 antibodies in the serum of normal individuals: A.G. Pockley, et al.; Immunol. Invest. 27, 367 (1998), Abstract;
Differential effects of axotomy on the in vivo synthesis of the stress-inducible and constitutive 70-kDa heat-shock proteins in rat dorsal root ganglia: B. Tedeschi & R.P. Ciavarra; Brain Res. Mol. Brain Res. 45, 199 (1997), Abstract;
A hitchhiker's guide to the human Hsp70 family: M. Tavaria, et al.; Cell Stress Chaperones 1, 23 (1996), Abstract;
Structural analysis of substrate binding by the molecular chaperone DnaK: X. Zhu, et al.; Science 272, 1606 (1996), Abstract;
Stress protein induction in skeletal muscle: comparison of laboratory models to naturally occurring hypertrophy: J.L. Kilgore, et al.; J. Appl. Physiol. 76, 598 (1994), Abstract;
Cellular and biochemical events in mammalian cells during and after recovery from physiological stress: W.J. Welch & J.P. Suhan; J. Cell Biol. 103, 2035 (1986), Abstract;

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