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Jurkat (heat shocked) , (cell lysate)

 
ADI-LYC-JK101-F 200 µg 92.00 USD
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Product Specification

Recommended Dilutions/Conditions:Western Blot (20µg/lane)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Source:Human
 
Concentration:2mg/ml
 
Formulation:Liquid. In SDS-PAGE sample buffer, pH 6.8, heated for 10 min. at 70°C and containing 40mM TRIS-HCl, 1% SDS, 50mM DTT, 7.5% glycerol, and 0.003% bromophenol blue.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The Jurkat T lymphocyte cell line (originally designated JM) was established from the peripheral blood of a 14 year old boy with T cell leukemia. Cellular stressors such as heat shock and oxidative stress result in the activation of heat shock protein gene expression by transcriptional regulators such as HSF1. Heat shock proteins play an important role in both normal cellular homeostasis as well as cell survival during and after cellular stress.
 

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