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Acetylated Lysine polyclonal antibody (HRP conjugate)

 
ADI-KAP-TF1203-E 100 µg 350.00 USD
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Product Specification

Alternative Name:AcK
 
Host:Rabbit
 
Immunogen:Acetylated KLH.
 
Species reactivity:Species independent
 
Applications:ELISA, IF, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (0.05-0.25µg/ml)
Immunohistochemistry (0.25-10µg/ml)
Western Blot (0.25-1.0µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Formulation:Liquid. In PBS, pH 7.0, containing 0.09% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Acetylation and methylation of lysine are important post-translational modifications that regulate numerous protein-protein and protein-DNA interactions. Lysine acetylation and methylation involves the transfer of acetylCoA, or one or more methyl groups, to the e-amino group of lysine by modifying enzymes and cofactors. Histones and transcription factors are the primary targets of lysine acetylation and methylation, with either modification capable of inducing gene silencing or expression due to differential regulation of cofactors. For example, varying degrees of mono-, di-, and tri-methylation or acetylation of histone H3 at lysine residue 9 are known to demark distinct chromatin regions during various states of gene activation (methylation) or repression (acetylation).
 
KAP-TF1203 WB
Western blot analysis of the acetylated histone from TSA treated (left) and non-treated (right) melanoma cell lysate with Acetylated Lysine pAb.
KAP-TF1203 IF
Immunofluorescent analysis of TSA treated (right- 100ng/ml, 24 hours) and non-treated (left) human MMRU melanoma cells using Acetylated Lysine pAb.
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KAP-TF1203 WB KAP-TF1203 IF

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Lysine
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Lysine Polyclonal antibody
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