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Caspase-7 monoclonal antibody (10-1-62)

 
ADI-AAM-137-C 25 µg 118.00 USD
 
ADI-AAM-137-E 100 µg 269.00 USD
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Product Specification

Clone:10-1-62
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human Caspase-7 protein (full length).
 
UniProt ID:P55210
 
Species reactivity:Human, Mouse, Rat
Hamster, Monkey
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Immunohistochemistry (paraffin sections)
Western Blot (1µg/ml, ECL)
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 10mM sodium phosphate, 50mM sodium chloride, and 0.1mM PMSF.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.
 
AAM-137-Antibody-Blot
ADI-AAM-137 IHC1
IHC Analysis: Human colon tissue stained with Caspase-7, mAb (10-1-62) at 10µg/ml.
ADI-AAM-137 IHC2
IHC Analysis: Human spleen tissue stained with Caspase-7, mAb (10-1-62) at 10µg/ml.
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AAM-137-Antibody-Blot ADI-AAM-137 IHC1 ADI-AAM-137 IHC2

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