Product Details
Clone: | 7-1-11 |
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Host: | Mouse |
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Isotype: | IgG2b |
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Immunogen: | Recombinant human Caspase-7 protein (full length) . |
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UniProt ID: | P55210 |
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Source: | Purified from mouse ascites. |
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Species reactivity: | Human, Mouse, Rat Dog, Hamster, Monkey, Porcine, Rabbit, Sheep
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Specificity: | Detects the unprocessed pro-caspase-7 (p35), caspase lacking the short amino-terminal pro-domain (p32) and fully processed caspase-7 (p19) in the apototic cell extract. |
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Applications: | IHC (PS), WB
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Recommended Dilutions/Conditions: | Western Blot (0.2µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Immunohistochemistry (paraffin sections) Western Blot (0.2µg/ml, ECL) Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 0.1mM PMSF. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death. |
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Regulatory Status: | RUO - Research Use Only |
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IHC Analysis: Human placenta tissue stained with Caspase-7, mAb (7-1-11) at 10µg/ml.
IHC Analysis: Human colon tissue stained with Caspase-7, mAb (7-1-11) at 10µg/ml.
Western blot analysis of Caspase-7, mAb (7-1-11) (Prod. No. ADI-AAM-127): Lane1: MW Marker; Lane 2: Jurkat (Prod. No.ADI-LYC-JK100); Lane 3: Jurkat plus Staurosporine; Lane 4: HeLa (Prod. No. ADI-LYC-HL100); Lane 5: HeLa plus Nocodazole; Lane 6: 3T3 (Prod. No. ADI-LYC-3T100); Lane 7: PC-12 (Prod. No. ADI-LYC-PC100).
IHC Analysis: Human spleen tissue stained with Caspase-7, mAb (7-1-11) at 10µg/ml.
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Product Literature References
A novel Hsp70 inhibitor prevents cell intoxication with the actin ADP-ribosylating Clostridium perfringens iota toxin: K. Ernst, et al.; Sci. Rep.
6, 20301 (2016),
Application(s): Incubated into membranes,
Abstract;
Full Text
Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c: J.M. Hardwick, et al. ; J. Biol. Chem.
274, 21155 (1999),
Application(s): WB using human samples,
Abstract;
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