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IMMUNOSET® Osteopontin (human), ELISA development set

 
ADI-960-142 10x96 wells 472.00 USD
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  • Quantitative - obtain fully quantitative statistically significant results
  • Sensitive - detect as little as 16pg/ml osteopontin compared to microgram levels in Western blot
  • Reliable - obtain accurate results with thoroughly tested reagents in multiple complex sample matrices
The IMMUNOSET® Osteopontin (human), EIA development set contains sufficient reagents for the development of 10 x 96 well EIA plates which provides analysis of ~400 samples in duplicate. Save time, money, and sample by developing your own EIA kits. Simply coat your plates and go!
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Product Specification

Alternative Name:OPN, Secreted phosphoprotein 1, Bone sialoprotein 1, SPP1
 
Sensitivity:0.016 ng/ml (range 0.03 - 1 ng/ml)
 
Assay Time:Plate Coating - Overnight + 1 hour; Assay - 3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human Osteopontin in culture supernatants, milk, and plasma.
 
Species reactivity:Human
 
Crossreactivity:Human OPN (100%) and <0.03%: mouse OPN, bovine OPN
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Capture antibody, Standard, Detection antibody, SA-HRP
 
Scientific Background:Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival. Plasma Osteopontin has been shown to be a positive indicator of colon and lung cancers as well as metastatic carcinomas. The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack. Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies.
 
UniProt ID:P10451
 

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