| BML-PW9300-0025 | 25 µl | 155.00 USD |  |
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| BML-PW9300-0100 | 100 µl | 450.00 USD | |
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| Alternative Name: | Proteasome subunit β type-7, Macropain chain Z |
| Clone: | MCP165 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Dinitrophenylated proteasomes. |
| UniProt ID: | Q99436 (human), P70195 (mouse) |
| Gene/Protein Identifier: | PSMB7 (gene name) |
| Source: | Purified from hybridoma tissue culture supernatant. |
| Species reactivity: | Human, Mouse |
| Specificity: | Recognizes the β2 subunit of the 20S proteasome. |
| Applications: | IHC, WB |
| Recommended Dilutions/Conditions: | Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations. Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue. Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa. |
| Purity Detail: | Protein G affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
| Use/Stability: | Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The hybridoma secreting the antibody to subunit β2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with dinitrophenylated proteasomes. The antibody (clone MCP165) has been characterised by one- and two dimensional Western blotting. The antibody has not been demonstrated to immunoprecipitate the native 20S proteasome. The immunoglobulin subclass is IgG1. |
| Technical Info/Product Notes: | Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins. |
| Regulatory Status: | RUO - Research Use Only |
Goat anti-mouse IgG, polyclonal antibody (AP conjugate) |
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| ELISA, WB | Print as PDF | ||||||
| ADI-SAB-101-J | 1 ml | 175.00 USD | |
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Proteasome 20S (human), (purified) |
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| High integrity preparation for use in proteasome research. | ||||||
| Isolated and purified from human erythrocytes. | Print as PDF | ||||||
| BML-PW8720-0050 | 50 µg | 379.00 USD | |
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Proteasome 20S β2 subunit monoclonal antibody (MCP168) |
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| Purified from hybridoma tissue culture supernatant., IHC, WB | Print as PDF | ||||||
| BML-PW8145-0025 | 25 µl | 160.00 USD | |
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| BML-PW8145-0100 | 100 µl | 481.00 USD | |
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Proteasome 20S β2i subunit polyclonal antibody |
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| IHC, WB | Print as PDF | ||||||
| BML-PW8210-0100 | 100 µl | 799.00 USD | |
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HeLa, (cell lysate) |
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| Human, WB | Print as PDF | ||||||
| ADI-LYC-HL100-F | 200 µg | 141.00 USD | |
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| Download as PDF | |
