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Proteasome 20S (human), (purified)

High integrity preparation for use in proteasome research.
BML-PW8720-0050 50 µg 379.00 USD
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Replaces Prod. #: ALX-202-047

Product Details

Source:Isolated and purified from human erythrocytes.
Formulation:Liquid. In 20mM TRIS-HCl, pH 7.2, containing 1mM EDTA, 1mM DTT, 1mM sodium azide and 50% glycerol.
Shipping:Dry Ice
Long Term Storage:-80°C
Use/Stability:20S Proteasome remains relatively stable and active so long as repetitive freeze-thaw cycles are avoided. It has been demonstrated that 20S proteasome undergoes only slow loss of activity when stored at 4°C for up to six months. If it is to be used on a regular basis, it is recommended that the material should be stored between 0°C-4°C during the duration of use. For longer term storage the material may be dispensed as single use aliquots and stored at -80°C.
Handling:Avoid freeze/thaw cycles.
Scientific Background:The 20S proteasome is the catalytic core of the 26S proteasome, the central protease of the ubiquitin pathway of protein degradation. Unlike the 26S proteasome, which degrades proteins in an ATP-dependent manner, the 20S proteasome is ATP-independent and only degrades entirely unfolded polypeptides.
Regulatory Status:RUO - Research Use Only

Product Literature References

Extracellular 20S proteasome secreted via microvesicles can degrade poorly folded proteins and inhibit Galectin-3 agglutination activity: A. Bonhoure, et al.; Traffic 23, 287 (2022), Abstract;
Genetic and pharmacologic proteasome augmentation ameliorates Alzheimer’s-like pathology in mouse and fly APP overexpression models: E.S. Chocron, et al.; Sci. Adv. 8, abk2252 (2022), Abstract;
Chemical Elicitors Induce Rare Bioactive Secondary Metabolites in Deep-Sea Bacteria under Laboratory Conditions: R. de Felício, et al.; Metabolites 11, 107 (2021), Abstract; Full Text
Cell cycle-dependent localization of the proteasome to chromatin: Y. Kito, et al.; Sci. Rep. 10, 5801 (2020), Abstract; Full Text
Vertically Aligned Graphene Prepared by Photonic Annealing for Ultrasensitive Biosensors: L. Wang, et al.; ACS Appl. Mater. Interfaces 12, 35328 (2020), Abstract;
Mass spectrometry-based absolute quantification of 20S proteasome status for controlled ex-vivo expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells: T. Menneteau, et al.; Mol. Cell. Proteomics 18, 744 (2019), Abstract;
Proline- and Arginine-Rich Peptides as Flexible Allosteric Modulators of Human Proteasome Activity: M. Gizynska, et al.; J. Med. Chem. 62, 359 (2019), Abstract; Full Text
Fast and high-resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM: S. Benaglia, et al.; Nat. Protoc. 13, 2890 (2018), Abstract;
Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders: S. Ugras, et al.; EBioMedicine 31, 307 (2018), Abstract; Full Text
The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function: H. Chen, et al.; Sci. Rep. 8, 8163 (2018), Abstract; Full Text
Nano-liquid chromatography-orbitrap MS -based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells: A. Valdés, et al.; Mol. Cell. Proteomics 16, 8 (2017), Abstract; Full Text
Reaction of complement factors and proteasomes in experimental encephalitis: S. Lange, et al.; J. Neurovirol. 23, 313 (2017), Abstract; Full Text
An informatic framework for decoding protein complexes by top-down mass spectrometry: O.S. Skinner, et al.; Nat. Methods. 13, 237 (2016), Abstract;
Oxidation and interaction of DJ-1 with 20S proteasome in the erythrocytes of early stage Parkinson’s disease patients: Y. Saito, et al.; Sci. Rep. 6, 30793 (2016), Application(s): Interaction analysis with oxidized DJ-1 recombinant protein, Abstract; Full Text
Total Sample Conditioning and Preparation of Nanoliter Volumes for Electron Microscopy: S.A. Arnold, et al.; ACS Nano 10, 4981 (2016), Application(s): Single-Particle test samples, Abstract; Full Text
Molecular mechanisms of Polyphyllin I-induced apoptosis and reversal of the epithelial-mesenchymal transition in human osteosarcoma cells: J. Chang, et al.; J. Ethnopharmacol. 170, 117 (2015), Abstract;
The presence of prolines in the flanking region of an immunodominant HIV-2 gag epitope influences the quality and quantity of the epitope generated: S. Jallow, et al.; Eur. J. Immunol. 45, 2232 (2015), Abstract; Full Text
PiZ Mouse Liver Accumulates Polyubiquitin Conjugates That Associate with Catalytically Active 26S Proteasomes: C.J. Haddock, et al.; PLoS One 9, e106371 (2014), Application(s): EM Analysis, Abstract; Full Text
Quinone-induced protein handling changes: implications for major protein handling systems in quinone-mediated toxicity: R. Xiong, et al.; Toxicol. Appl. Pharmacol. 280, 285 (2014), Application(s): Assay, Abstract;
Differential Roles of Proteasome and Immunoproteasome Regulators Pa28αβ, Pa28γ and Pa200 in the Degradation of Oxidized Proteins: A.M. Pickering, et al.; Arch. Biochem. Biophys. 523, 181 (2012), Application(s): Assay, Abstract; Full Text
Pivotal Advance: Protein synthesis modulates responsiveness of differentiating and malignant plasma cells to proteasome inhibitors: S. Censi, et al.; J. Leukoc. Biol. 92, 921 (2012), Abstract;
Aaptamine, an alkaloid from the sponge Aaptos suberitoides, functions as a proteasome inhibitor: S. Tsukamoto, et al.; Bioorg. Med. Chem. Lett. 20, 3341 (2010), Abstract;

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