Product Details
Alternative Name: | CD95, APO-1, TNFRSF 6 |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to a portion of mouse Fas. |
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UniProt ID: | P25446 |
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GenBank ID: | M83649 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human, Mouse, Rat
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Applications: | IHC, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (1:50) Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~45kDa by Western blot. |
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Purity Detail: | Peptide affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Fas/APO-1/CD95 is a 36 kDa cell surface type I-membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas belongs to the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells. Both Fas and TNFR contain a 70 amino acid cytoplasmic “death domain” responsible for transmitting signals for apoptosis. Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long forms of FLIP. Insertion of an early transposable element in intron 2 of the Fas gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting the involvement of Fas in clonal deletion of autoreactive T cells. |
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Regulatory Status: | RUO - Research Use Only |
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Western Blot Analysis of Fas pAb : Lane 1: Rat Brain Tissue Extract, Lane 2: Mouse Brain Tissue Extract.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human spleen tissue stained with Fas polyclonal antibody, (Prod. No. ADI-AAP-221) at 10µg/ml.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human colon tumor tissue stained with Fas polyclonal antibody, (Prod. No. ADI-AAP-221) at 10µg/ml.
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Product Literature References
Sulforaphane-induced Cell Death in Human Prostate Cancer Cells Is Initiated by Reactive Oxygen Species: S.V. Singh, et al.; J. Biol. Chem.
280, 19911 (2005),
Abstract;
Key apoptotic pathways for heat-induced programmed germ cell death in the testis: R.S. Swerdloff, et al. ; Endocrinology
144, 3167 (2003),
Application(s): WB using rat samples,
Abstract;
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